IL-10-driven immunoglobulin production by B lymphocytes from IgA-deficient individuals correlates to infection proneness.

• In search for a possible explanation of the phenotypic heterogeneity in IgA deficiency, we studied the function of B cells from IgA-deficient (IgAd) individuals. Two groups of IgAd individuals, one frequently infected and one clinically apparently healthy, as well as normal controls, were studied. Peripheral blood mononuclear cells (PBMC) and B cells from IgAd individuals and controls were cultured with Staphylococcus aureus Cowan I strain and with anti-CD40 MoAb presented on the CD32-transfected fibroblast cell line in the presence of IL-10. In this experimental system PBMC and B cells from the infection-prone IgAd individuals produced only minute amounts of IgA. In contrast, PBMC and B cells from healthy IgAd subjects secreted significantly more IgA1 and IgA2 in comparison with infection-prone IgAd patients (P < 0.05). These data suggest that the abnormalities of B cell differentiation in IgAd could be of heterogeneous origin. Thus, whereas in healthy IgAd subjects IgA production may be efficiently up-regulated in vitro by addition of IL-10 to CD40-activated B cell culture, the corresponding B cell differentiation does not occur in infection-prone IgAd patients. These observations provide a conceptual framework for phenotypic heterogeneity in IgAd subjects.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Nyckelord

Adult

Antibodies

Monoclonal

immunology

Antibody Formation

B-Lymphocytes

immunology

CD40 Antigens

immunology

Cells

Cultured

Coculture Techniques

Culture Media

analysis

Dysgammaglobulinemia

immunology

Enzyme-Linked Immunosorbent Assay

Female

Fibroblasts

Humans

IgA Deficiency

immunology

Immunoglobulin A

analysis

biosynthesis

Immunoglobulin G

analysis

biosynthesis

Immunoglobulin M

analysis

biosynthesis

Interleukin-10

genetics

immunology

Leukocytes

Mononuclear

immunology

Lymphocyte Activation

Male

Middle Aged

Recombinant Proteins

immunology

Respiratory Tract Infections

immunology

Staphylococcal Infections

immunology

Staphylococcus aureus

Transfection

Up-Regulation

Publikations- och innehållstyp

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art (ämneskategori)