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Galectin-3 enhances...
Galectin-3 enhances monocyte-derived macrophage efferocytosis of apoptotic granulocytes in asthma
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Erriah, M. (författare)
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Pabreja, K. (författare)
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Fricker, M. (författare)
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Baines, K. J. (författare)
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Donnelly, L. E. (författare)
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- Bylund, Johan, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
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- Karlsson, Anna, 1967 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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Simpson, J. L. (författare)
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Rlsson Sr, Journal Of Biological Chemistry V. P. (författare)
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(creator_code:org_t)
- 2019-01-03
- 2019
- Engelska.
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Ingår i: Respiratory Research. - : Springer Science and Business Media LLC. - 1465-993X. ; 20
- Relaterad länk:
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BackgroundGalectin-3 is a 32kDa protein secreted by macrophages involved in processes such as cell activation, chemotaxis and phagocytosis. Galectin-3 has previously been shown to improve the ability of airway macrophages to ingest apoptotic cells (efferocytosis) in chronic obstructive pulmonary disease (COPD) and may be of interest in non-eosinophilic asthma (NEA) which is also characterised by impaired efferocytosis. It was hypothesised that the addition of exogenous galectin-3 to monocyte-derived macrophages (MDMs) derived from donors with NEA would enhance their ability to engulf apoptotic granulocytes.MethodsEligible non-smoking adults with asthma (n=19), including 7 with NEA and healthy controls (n=10) underwent a clinical assessment, venepuncture and sputum induction. MDMs were co-cultured with apoptotic granulocytes isolated from healthy donors with or without exogenous recombinant galectin-3 (50g/mL) and efferocytosis was assessed by flow cytometry. Galectin-3 expression and localisation in MDMs was visualised by immunofluorescence staining and fluorescence microscopy. Galectin-3, interleukin (IL)-6 and CXCL8 secretion were measured in cell culture supernatants by ELISA and cytometric bead array.ResultsBaseline efferocytosis (mean (standard deviation)) was lower in participants with asthma (33.2 (+/- 17.7)%) compared with healthy controls (45.3 (+/- 15.9)%; p=0.081). Efferocytosis did not differ between the participants with eosinophilic asthma (EA) (31.4 (+/- 19.2)%) and NEA (28.7 (+/- 21.5)%; p=0.748). Addition of galectin-3 significantly improved efferocytosis in asthma, particularly in NEA (37.8 (+/- 18.1)%) compared with baseline (30.4 (+/- 19.7)%; p=0.012). Efferocytosis was not associated with any of the clinical outcomes but was negatively correlated with sputum macrophage numbers (Spearman r=-0.671; p=0.017). Galectin-3 was diffusely distributed in most MDMs but formed punctate structures in 5% of MDMs. MDM galectin-3 secretion was lower in asthma (9.99 (2.67, 15.48) ng/mL) compared with the healthy controls (20.72 (11.28, 27.89) ng/mL; p=0.044) while IL-6 and CXCL8 levels were similar.Conclusions p id=Par4 Galectin-3 modulates macrophage function in asthma, indicating a potential role for galectin-3 to reverse impaired efferocytosis in NEA.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Asthma
- Galectin-3
- Macrophage
- Neutrophil
- Efferocytosis
- Inflammation
- expression
- phagocytosis
- clearance
- kinase
- cells
- Respiratory System
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Erriah, M.
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Pabreja, K.
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Fricker, M.
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Baines, K. J.
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Donnelly, L. E.
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Bylund, Johan, 1 ...
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visa fler...
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Karlsson, Anna, ...
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Simpson, J. L.
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Rlsson Sr, Journ ...
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Respiratory Rese ...
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Göteborgs universitet