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Skeletonema marinoi...
Skeletonema marinoi as a new genetic model for marine chain-forming diatoms
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- Johansson, Oskar N., 1984 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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- Töpel, Mats H., 1973 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Department of marine sciences
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- Pinder, Matthew I. M., 1990 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Department of marine sciences
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- Kourtchenko, Olga, 1980 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Department of marine sciences
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- Blomberg, Anders, 1956 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Godhe, Anna, 1967 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Department of marine sciences
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- Clarke, Adrian K, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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(creator_code:org_t)
- 2019-04-02
- 2019
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
- Relaterad länk:
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Diatoms are ubiquitous primary producers in marine ecosystems and freshwater habitats. Due to their complex evolutionary history, much remains unknown about the specific gene functions in diatoms that underlie their broad ecological success. In this study, we have genetically transformed the centric diatom Skeletonema marinoi, a dominant phytoplankton species in temperate coastal regions. Transformation of S. marinoi is the first for a true chain-forming diatom, with the random genomic integration via non homologous recombination of a linear DNA construct expressing the resistance gene to the antibiotic zeocin. A set of molecular tools were developed for reliably identifying the genomic insertion site within each transformant, many of which disrupt recognizable genes and constitute null or knock-down mutations. We now propose S. marinoi as a new genetic model for marine diatoms, representing true chain-forming species that play a central role in global photosynthetic carbon sequestration and the biogeochemical cycling of silicates and various nutrients, as well as having potential biotechnological applications.
Ämnesord
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
Nyckelord
- phaeodactylum-tricornutum
- thalassiosira-pseudonana
- nuclear
- transformation
- evolution
- genome
- reveals
- ocean
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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