Sökning: onr:"swepub:oai:gup.ub.gu.se/281358" >
The endogenous hydr...
The endogenous hydrogen sulfide generating system regulates ovulation
-
Estienne, A. (författare)
-
Portela, V. M. (författare)
-
Choi, Y. (författare)
-
visa fler...
-
Zamberlam, G. (författare)
-
Boerboom, D. (författare)
-
Roussel, V. (författare)
-
Meinsohn, M. C. (författare)
-
- Brännström, Mats, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology
-
Curry, T. E. (författare)
-
Jo, M. (författare)
-
Price, C. A. (författare)
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- Engelska.
-
Ingår i: Free Radical Biology and Medicine. - : Elsevier BV. - 0891-5849. ; 138, s. 43-52
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The generation of free-radicals such as nitric oxide has been implicated in the regulation of ovarian function, including ovulation. Tissues that generate nitric oxide typically generate another free-radical gas, hydrogen sulfide (H2S), although little is known about the role of H2S in ovarian function. The hypothesis of this study was that H2S regulates ovulation. Treatment with luteinizing hormone (LH) increased the levels of mRNA and protein of the H2S generating enzyme cystathionine.-lyase (CTH) in granulosa cells of mice and humans in vivo and in vitro. Pharmacological inhibition of H2S generating enzymes reduced the number of follicles ovulating in mice in vivo and in vitro, and this inhibitory action was reversed by cotreatment with a H2S donor. Addition of a H2S donor to cultured mouse granulosa cells increased basal and LH-dependent abundance of mRNA encoding amphiregulin, betacellulin and tumor necrosis alpha induced protein 6, proteins important for cumulus expansion and follicle rupture. Inhibition of CTH activity reduced abundance of mRNA encoding matrix metalloproteinase-2 and -9 and tissue-type plasminogen activator, and cotreatment with the H2S donor increased the levels of these mRNA above those stimulated by LH alone. We conclude that the H2S generating system plays an important role in the propagation of the preovulatory cascade and rupture of the follicle at ovulation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Nyckelord
- Ovary
- Granulosa cells
- Follicle
- Ovulation
- cystathionine beta-synthase
- nitric-oxide synthase
- activator
- gene-function
- plasminogen-activator
- granulosa-cells
- expression
- mice
- ovarian
- prostaglandins
- progesterone
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Estienne, A.
-
Portela, V. M.
-
Choi, Y.
-
Zamberlam, G.
-
Boerboom, D.
-
Roussel, V.
-
visa fler...
-
Meinsohn, M. C.
-
Brännström, Mats ...
-
Curry, T. E.
-
Jo, M.
-
Price, C. A.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Reproduktionsmed ...
- Artiklar i publikationen
-
Free Radical Bio ...
- Av lärosätet
-
Göteborgs universitet