Sökning: onr:"swepub:oai:gup.ub.gu.se/284853" > Characterization of...
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000 | 08354naa a2200997 4500 | |
001 | oai:gup.ub.gu.se/284853 | |
003 | SwePub | |
008 | 240910s2019 | |||||||||||000 ||eng| | |
009 | oai:research.chalmers.se:ddbd8889-e381-4ef8-9903-2d54c0242bcb | |
009 | oai:DiVA.org:su-174941 | |
009 | oai:prod.swepub.kib.ki.se:142647769 | |
009 | oai:DiVA.org:uu-393235 | |
009 | oai:DiVA.org:ri-39975 | |
024 | 7 | a https://gup.ub.gu.se/publication/2848532 URI |
024 | 7 | a https://doi.org/10.3390/bios90301092 DOI |
024 | 7 | a https://research.chalmers.se/publication/5127802 URI |
024 | 7 | a https://research.chalmers.se/publication/5126802 URI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1749412 URI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1426477692 URI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3932352 URI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-399752 URI |
040 | a (SwePub)gud (SwePub)cthd (SwePub)sud (SwePub)kid (SwePub)uud (SwePub)ri | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Sepehri, Sobhan,d 1986u Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden,Chalmers University of Technology, Sweden4 aut0 (Swepub:ri)sobhanse@ri.se |
245 | 1 0 | a Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay |
264 | c 2019-09-17 | |
264 | 1 | b MDPI AG,c 2019 |
520 | a The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes. | |
650 | 7 | a NATURVETENSKAPx Fysik0 (SwePub)1032 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Physical Sciences0 (SwePub)1032 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemix Materialkemi0 (SwePub)104032 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Materials Chemistry0 (SwePub)104032 hsv//eng |
650 | 7 | a NATURVETENSKAPx Fysikx Den kondenserade materiens fysik0 (SwePub)103042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Physical Sciencesx Condensed Matter Physics0 (SwePub)103042 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Nanoteknik0 (SwePub)2102 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Nano-technology0 (SwePub)2102 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng |
653 | a magnetic nanoparticle | |
653 | a bioassay | |
653 | a differential homogenous magnetic assay | |
653 | a immobilization | |
653 | a binding | |
653 | a signal amplification | |
653 | a padlock probes | |
653 | a bacterial-DNA | |
653 | a nanobeads | |
653 | a Chemistry | |
653 | a biomolecular dynamics | |
653 | a Teknisk fysik med inriktning mot nanoteknologi och funktionella material | |
700 | 1 | a Agnarsson, Björn,d 1977u Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Phys, Gothenburg, Sweden,Chalmers University of Technology, Sweden4 aut0 (Swepub:cth)bjornag |
700 | 1 | a Zardán Gómez de la Torre, Teresau Uppsala universitet,Nanoteknologi och funktionella material,Uppsala University, Sweden4 aut0 (Swepub:uu)terza442 |
700 | 1 | a Schneiderman, Justin F.,d 1979u Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology,Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden; Univ Gothenburg, MedTech West, Gothenburg, Sweden; Univ Gothenburg, Inst Neurosci & Physiol, Gothenburg, Sweden,Chalmers University of Technology, Sweden; University of Gothenburg, Sweden4 aut0 (Swepub:cth)justins |
700 | 1 | a Blomgren, Jakobu RISE,Acreo4 aut0 (Swepub:ri)JakobBl@ri.se |
700 | 1 | a Jesorka, Aldo,d 1967u Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden4 aut0 (Swepub:cth)aldo |
700 | 1 | a Johansson, Christer,d 1964u RISE,Acreo4 aut0 (Swepub:ri)christer.johansson@ri.se |
700 | 1 | a Nilsson, Matsu Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, Solna, Sweden,Stockholm University, Sweden4 aut0 (Swepub:su)matsn |
700 | 1 | a Albert, J.u Karolinska Institutet,Karolinska Univ Hosp, Dept Clin Microbiol, Stockholm, Sweden; Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden,Karolinska University Hospital, Sweden; Karolinska Institute, Sweden4 aut |
700 | 1 | a Strömme, Maria,d 1970-u Uppsala universitet,Nanoteknologi och funktionella material,Uppsala University, Sweden4 aut0 (Swepub:uu)mst26208 |
700 | 1 | a Winkler, Dag,d 1957u Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden,Chalmers University of Technology, Sweden4 aut0 (Swepub:cth)winkler1 |
700 | 1 | a Kalaboukhov, Alexei,d 1975u Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden,Chalmers University of Technology, Sweden4 aut0 (Swepub:cth)ascry |
700 | 1 | a Schneiderman, Justin E.4 aut |
710 | 2 | a Chalmers tekniska högskolab Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden4 org |
773 | 0 | t Biosensors-Baseld : MDPI AGg 9:3q 9:3 |
773 | 0 | t Biosensorsd : MDPI AGg 9:3q 9:3x 2079-6374 |
856 | 4 | u https://www.mdpi.com/2079-6374/9/3/109/pdf |
856 | 4 | u https://research.chalmers.se/publication/512780/file/512780_Fulltext.pdfx primaryx freey FULLTEXT |
856 | 4 | u https://doi.org/10.3390/bios9030109y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1352146/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://gup.ub.gu.se/publication/284853 |
856 | 4 8 | u https://doi.org/10.3390/bios9030109 |
856 | 4 8 | u https://research.chalmers.se/publication/512780 |
856 | 4 8 | u https://research.chalmers.se/publication/512680 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-174941 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:142647769 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393235 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-39975 |
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