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Ovarian Blood Sampl...
Ovarian Blood Sampling Identifies Junction Plakoglobin as a Novel Biomarker of Early Ovarian Cancer
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Weiland, F. (författare)
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Lokman, N. A. (författare)
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Klingler-Hoffmann, M. (författare)
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Jobling, T. (författare)
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Stephens, A. N. (författare)
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- Sundfeldt, Karin, 1962 (författare)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology
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Hoffmann, P. (författare)
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Oehler, M. K. (författare)
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(creator_code:org_t)
- 2020-09-25
- 2020
- Engelska.
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Ingår i: Frontiers in Oncology. - : Frontiers Media SA. - 2234-943X. ; 10
- Relaterad länk:
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https://www.frontier...
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https://gup.ub.gu.se...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Ovarian cancer is the most lethal gynecologic malignancy. Early detection would improve survival, but an effective diagnostic test does not exist. Novel biomarkers for early ovarian cancer diagnosis are therefore warranted. We performed intraoperative blood sampling from ovarian veins of stage I epithelial ovarian carcinomas and analyzed the serum proteome. Junction plakoglobin (JUP) was found to be elevated in venous blood from ovaries with malignancies when compared to those with benign disease. Peripheral plasma JUP levels were validated by ELISA in a multicenter international patient cohort. JUP was significantly increased in FIGO serous stage IA+B (1.97-fold increase; p < 0.001; n = 20), serous stage I (2.09-fold increase; p < 0.0001; n = 40), serous stage II (1.81-fold increase, p < 0.001, n = 23) and serous stage III ovarian carcinomas (1.98-fold increase; p < 0.0001; n = 34) vs. normal controls (n = 109). JUP plasma levels were not increased in early stage breast cancer (p = 0.122; n = 12). In serous ovarian cancer patients, JUP had a sensitivity of 85% in stage IA+B and 60% in stage IA-C, with specificities of 76 and 94%, respectively. A logistic regression model of JUP and Cancer Antigen 125 (CA125) revealed a sensitivity of 70% for stage IA+B and 75% for stage IA-C serous carcinomas at 100% specificity. Our novel ovarian blood sampling – proteomics approach identified JUP as a promising new biomarker for epithelial ovarian cancer, which in combination with CA125 might fulfill the test criteria for ovarian cancer screening. © Copyright © 2020 Weiland, Lokman, Klingler-Hoffmann, Jobling, Stephens, Sundfeldt, Hoffmann and Oehler.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- abundance protein depletion
- biomarker
- early diagnosis
- junction plakoglobin
- mass spectrometry
- ovarian cancer
- proteomics
- saturation labeling 2D-DIGE
- biological marker
- plakoglobin
- adult
- Article
- biotinylation
- blood sampling
- cancer diagnosis
- cancer screening
- cancer staging
- clear cell carcinoma
- controlled study
- endometrioid carcinoma
- enzyme linked immunosorbent assay
- female
- human
- image analysis
- liquid chromatography-mass spectrometry
- major clinical study
- matrix assisted laser desorption ionization time of flight mass spectrometry
- middle aged
- ovary cancer
- ovary cyst
- prevalence
- prospective study
- protein analysis
- tandem mass spectrometry
- two dimensional difference gel electrophoresis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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