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The secretion of cy...
The secretion of cytokines by peripheral blood mononuclear cells of patients with periodontitis and healthy controls when exposed to H2S
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- Basic, Amina (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 3,Institute of Odontology, Section 3
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Serino, G. (författare)
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- Leonhardt, Åsa, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
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- Dahlén, Gunnar, 1944 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 3,Institute of Odontology, Section 3
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- Bylund, Johan, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 3,Institute of Odontology, Section 3
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(creator_code:org_t)
- 2021-08-10
- 2021
- Engelska.
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Ingår i: Journal of Oral Microbiology. - : Informa UK Limited. - 2000-2297. ; 13:1
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Hydrogen sulfide(H2S) is a bacterial metabolite produced as a result of bacterial growth in subgingival pockets, suggested to partake in the pathogenesis of periodontitis. H2S has previously been shown to induce the secretion of the pro-inflammatory cytokines IL-1 beta and IL-18 via the NLRP3 inflammasome in monocytes. Objective: To investigate the non-NLRP3 inflammasome-dependent immunological response of human peripheral blood mononuclear cells (PBMCs) of periodontitis patients and healthy controls exposed to H2S in vitro. Methods: PBMCs of periodontitis patients(N = 31) and healthy controls(N = 32) were exposed to 1 mM sodium hydrosulfide (NaHS) at 37 degrees C for 24 h and the secretion of cytokines was compared to resting cells. TNF-alpha, IFN-gamma, IL-6, IL-8, IL-12p40, IL-12p70, IL-17, MCP-1, and IL-1Ra secretions were measured with Bio-Plex Pro (TM) Human Cytokine Assay. Results: H2S triggered the secretion of the pro-inflammatory IFN-gamma, IL-6, IL-17, TNF-alpha, IL-12p40, and IL-12p70, while the reverse was seen for IL-1Ra. In addition, a higher basal secretion of IFN-gamma, IL-6, IL-12p70, IL-17 and MCP-1 was seen from PBMCs of periodontitis patients compared to healthy controls. Conclusion: The bacterial metabolite H2S triggers the secretion of pro-inflammatory cytokines from PBMCs and may thus have a prominent role in the host-bacteria interplay in periodontitis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- H2S
- periodontitis
- PBMCs
- TNF-alpha
- IFN-gamma
- IL-6
- IL-8
- IL-12p40
- IL-12p70
- IL-17
- MCP-1
- IL-1Ra
- crevicular fluid levels
- necrosis-factor-alpha
- hydrogen-sulfide
- methyl
- mercaptan
- diseases
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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