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Modelling human liv...
Modelling human liver fibrosis in the context of non-alcoholic steatohepatitis using a microphysiological system
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Kostrzewski, T. (författare)
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Snow, S. (författare)
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Battle, A. L. (författare)
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visa fler...
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Peel, S. (författare)
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Ahmad, Z. (författare)
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Basak, J. (författare)
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Surakala, M. (författare)
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Bornot, A. (författare)
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Lindgren, J. (författare)
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Ryaboshapkina, M. (författare)
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Clausen, M. (författare)
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- Lindén, Daniel, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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Maass, C. (författare)
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Young, L. M. (författare)
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Corrigan, A. (författare)
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Ewart, L. (författare)
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Hughes, D. (författare)
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(creator_code:org_t)
- 2021-09-15
- 2021
- Engelska.
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 4:1
- Relaterad länk:
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Kostrzewski et al. introduce an in vitro microphysiological model of non-alcoholic steatohepatitis (NASH), consisting of co-cultured primary human liver cells. The authors characterised the transcriptomic, inflammatory and fibrotic phenotype of the model and show that major features of NASH can be recapitulated and therapeutic interventions mimicked. Non-alcoholic steatohepatitis (NASH) is a common form of chronic liver disease characterised by lipid accumulation, infiltration of immune cells, hepatocellular ballooning, collagen deposition and liver fibrosis. There is a high unmet need to develop treatments for NASH. We have investigated how liver fibrosis and features of advanced clinical disease can be modelled using an in vitro microphysiological system (MPS). The NASH MPS model comprises a co-culture of primary human liver cells, which were cultured in a variety of conditions including+/- excess sugar, fat, exogenous TGF beta or LPS. The transcriptomic, inflammatory and fibrotic phenotype of the model was characterised and compared using a system biology approach to identify conditions that mimic more advanced clinical disease. The transcriptomic profile of the model was shown to closely correlate with the profile of patient samples and the model displayed a quantifiable fibrotic phenotype. The effects of Obeticholic acid and Elafibranor, were evaluated in the model, as wells as the effects of dietary intervention, with all able to significantly reduce inflammatory and fibrosis markers. Overall, we demonstrate how the MPS NASH model can be used to model different aspects of clinical NASH but importantly demonstrate its ability to model advanced disease with a quantifiable fibrosis phenotype.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- term-follow-up
- obeticholic acid
- preclinical models
- disease
- pathogenesis
- multicenter
- progression
- cirrhosis
- patterns
- agonist
- Life Sciences & Biomedicine - Other Topics
- Science & Technology - Other
- Topics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Kostrzewski, T.
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Snow, S.
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Battle, A. L.
-
Peel, S.
-
Ahmad, Z.
-
Basak, J.
-
visa fler...
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Surakala, M.
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Bornot, A.
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Lindgren, J.
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Ryaboshapkina, M ...
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Clausen, M.
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Lindén, Daniel, ...
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Maass, C.
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Young, L. M.
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Corrigan, A.
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Ewart, L.
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Hughes, D.
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
- Artiklar i publikationen
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Communications B ...
- Av lärosätet
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Göteborgs universitet