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Simultaneous Counti...
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He, XiulanGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
(författare)
Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening
- Artikel/kapitelEngelska2022
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Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/314086
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https://gup.ub.gu.se/publication/314086URI
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https://doi.org/10.1002/anie.202116217DOI
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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We report the discovery that in the presence of chaotropic anions (SCN-) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN-.
Ämnesord och genrebeteckningar
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NATURVETENSKAP Kemi hsv//swe
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NATURAL SCIENCES Chemical Sciences hsv//eng
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Amperometry
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Chaotropic Effect
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Hofmeister Series
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Large Dense-Core
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Vesicles
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Vesicle Opening
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secretory granule
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catecholamine content
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vesicular exocytosis
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ph
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cells
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size
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transmitters
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populations
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mechanisms
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kinetics
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Chemistry
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xewian
(författare)
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Göteborgs universitetInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Angewandte Chemie-International Edition: Wiley61:151433-78511521-3773
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