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Production and HPLC...
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Persson, AndreaGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Sahlgrenska Academy
(författare)
Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
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2021-10-10
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New York, NY :Springer,2022
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/314493
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https://gup.ub.gu.se/publication/314493URI
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https://doi.org/10.1007/978-1-0716-1398-6_15DOI
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https://lup.lub.lu.se/record/b73947bb-ba9c-4169-84b3-633297ff37d2URI
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Ämneskategori:vet swepub-contenttype
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Ämneskategori:kap swepub-publicationtype
Anmärkningar
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Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Tykesson, EmilLund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lung Biology,Lund University Research Groups(Swepub:lu)med-elt
(författare)
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Ellervik, UlfLund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Lung Biology,Lund University Research Groups,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ok2-uel
(författare)
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Mani, KatrinLund University,Lunds universitet,Glykobiologigruppen,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Glycobiology,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments(Swepub:lu)medk-kma
(författare)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för laboratoriemedicin
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Glycosaminoglycans. Methods in Molecular Biology, vol 2303. Balagurunathan K., Nakato H., Desai U., Saijoh Y. (eds)New York, NY : Springer, s. 173-1821064-37451940-60299781071613986
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