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Microbial and human...
Microbial and human transcriptome in vaginal fluid at midgestation: Association with spontaneous preterm delivery.
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- Wikström, Tove (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology,University of Gothenburg
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- Abrahamsson, Sanna (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Bioinformatics,Core Facilities, Bioinformatics,University of Gothenburg
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- Bengtsson-Palme, Johan, 1985 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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- Ek, C. Joakim (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,University of Gothenburg
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- Kuusela, Pihla (författare)
- Södra Älvsborgs Sjukhus (SÄS),Södra Älvsborg Hospital (SÄS)
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- Rekabdar, Elham, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Genomics,Core Facilities, Genomics,University of Gothenburg
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- Lindgren, Peter (författare)
- Karolinska Institutet
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- Wennerholm, Ulla-Britt, 1948 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology,University of Gothenburg
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- Jacobsson, Bo, 1960 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology,University of Gothenburg
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- Valentin, Lil (författare)
- Skånes universitetssjukhus (SUS),Skåne University Hospital,Lunds universitet,Lund University
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- Hagberg, Henrik, 1955 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology,University of Gothenburg
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(creator_code:org_t)
- 2022-09-14
- 2022
- Engelska.
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Ingår i: Clinical and translational medicine. - : Wiley. - 2001-1326. ; 12:9
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Abstract
Ämnesord
Stäng
- Intrauterine infection and inflammation caused by microbial transfer from the vagina are believed to be important factors causing spontaneous preterm delivery (PTD). Multiple studies have examined the relationship between the cervicovaginal microbiome and spontaneous PTD with divergent results. Most studies have applied a DNA-based assessment, providing information on the microbial composition but not transcriptional activity. A transcriptomic approach was applied to investigate differences in the active vaginal microbiome and human transcriptome at midgestation between women delivering spontaneously preterm versus those delivering at term.Vaginal swabs were collected in women with a singleton pregnancy at 18 + 0 to 20 + 6 gestational weeks. For each case of spontaneous PTD (delivery<37 + 0weeks) two term controls were randomized (39 + 0 to 40 + 6weeks). Vaginal specimens were subject to sequencing of both human and microbial RNA. Microbial reads were taxonomically classified using Kraken2 and RefSeq as a reference. Statistical analyses were performed using DESeq2. GSEA and HUMAnN3 were used for pathway analyses.We found 17 human genes to be differentially expressed (false discovery rate, FDR<0.05) in the preterm group (n =48) compared to the term group (n =96). Gene expression of kallikrein-2 (KLK2), KLK3 and four isoforms of metallothioneins 1 (MT1s) was higher in the preterm group (FDR<0.05). We found 11 individual bacterial species to be differentially expressed (FDR<0.05), most with a low occurrence. No statistically significant differences in bacterial load, diversity or microbial community state types were found between the groups.In our mainly white population, primarily bacterial species of low occurrence were differentially expressed at midgestation in women who delivered preterm versus at term. However, the expression of specific human transcripts including KLK2, KLK3 and several isoforms of MT1s was higher in preterm cases. This is of interest, because these genes may be involved in critical inflammatory pathways associated with spontaneous PTD.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Nyckelord
- Bacteria
- Bodily Secretions
- Body Fluids
- Female
- Humans
- Infant
- Newborn
- Pregnancy
- Premature Birth
- epidemiology
- genetics
- Transcriptome
- genetics
- Vagina
- microbiology
- transcriptome
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Wikström, Tove
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Abrahamsson, San ...
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Bengtsson-Palme, ...
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Ek, C. Joakim
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Kuusela, Pihla
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Rekabdar, Elham, ...
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visa fler...
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Lindgren, Peter
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Wennerholm, Ulla ...
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Jacobsson, Bo, 1 ...
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Valentin, Lil
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Hagberg, Henrik, ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Reproduktionsmed ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Mikrobiologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Genetik
- Artiklar i publikationen
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Clinical and tra ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola
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Karolinska Institutet