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Inference of gliobl...
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Rosén, EmilUppsala universitet,Uppsala University,Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.
(författare)
Inference of glioblastoma migration and proliferation rates using single time-point images
- Artikel/kapitelEngelska2023
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LIBRIS-ID:oai:gup.ub.gu.se/327591
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https://gup.ub.gu.se/publication/327591URI
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https://doi.org/10.1038/s42003-023-04750-0DOI
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https://research.chalmers.se/publication/535487URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-502114URI
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Cancer cell migration is a driving mechanism of invasion in solid malignant tumors. Anti-migratory treatments provide an alternative approach for managing disease progression. However, we currently lack scalable screening methods for identifying novel anti-migratory drugs. To this end, we develop a method that can estimate cell motility from single end-point images in vitro by estimating differences in the spatial distribution of cells and inferring proliferation and diffusion parameters using agent-based modeling and approximate Bayesian computation. To test the power of our method, we use it to investigate drug responses in a collection of 41 patient-derived glioblastoma cell cultures, identifying migration-associated pathways and drugs with potent anti-migratory effects. We validate our method and result in both in silico and in vitro using time-lapse imaging. Our proposed method applies to standard drug screen experiments, with no change needed, and emerges as a scalable approach to screen for anti-migratory drugs. The spatial positioning of cultured glioblastoma cells is used to estimate cell motility and drug effects from single end-point images in vitro.
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Mangukiya, Hitesh,ResearcherUppsala universitet,Uppsala University,Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.(Swepub:uu)manhi588
(författare)
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Elfineh, LioudmilaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.(Swepub:uu)liouelfi
(författare)
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Stockgard, R.Uppsala universitet,Neuroonkologi och neurodegeneration,Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.
(författare)
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Krona, Cecilia,1976-Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.(Swepub:uu)ceckr429
(författare)
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Gerlee, Philip,1980Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Math Sci, Gothenburg, Sweden.;Univ Gothenburg, Math Sci, Gothenburg, Sweden.(Swepub:cth)gerlee
(författare)
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Nelander, SvenUppsala universitet,Science for Life Laboratory, SciLifeLab,Neuroonkologi och neurodegeneration,Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.(Swepub:uu)svene843
(författare)
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Uppsala universitetUppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.
(creator_code:org_t)
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Ingår i:Communications Biology: Springer Nature6:12399-3642
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