Sökning: onr:"swepub:oai:gup.ub.gu.se/329177" >
The salivary proteo...
The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach
-
van Leeuwen, S. J. M. (författare)
-
Proctor, G. B. (författare)
-
Staes, A. (författare)
-
visa fler...
-
Laheij, Amga (författare)
-
Potting, C. M. J. (författare)
-
Brennan, M. T. (författare)
-
- von Bültzingslöwen, Inger, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
-
Rozema, F. R. (författare)
-
Hazenberg, M. D. (författare)
-
Blijlevens, N. M. A. (författare)
-
Raber-Durlacher, J. E. (författare)
-
Huysmans, Mcdnjm (författare)
-
visa färre...
-
(creator_code:org_t)
- 2023
- 2023
- Engelska.
-
Ingår i: Bmc Oral Health. - 1472-6831. ; 23:1
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- BackgroundOral mucositis is a frequently seen complication in the first weeks after hematopoietic stem cell transplantation recipients which can severely affects patients quality of life. In this study, a labelled and label-free proteomics approach were used to identify differences between the salivary proteomes of autologous hematopoietic stem cell transplantation (ASCT) recipients developing ulcerative oral mucositis (ULC-OM; WHO score & GE; 2) or not (NON-OM).MethodsIn the TMT-labelled analysis we pooled saliva samples from 5 ULC-OM patients at each of 5 timepoints: baseline, 1, 2, 3 weeks and 3 months after ASCT and compared these with pooled samples from 5 NON-OM patients. For the label-free analysis we analyzed saliva samples from 9 ULC-OM and 10 NON-OM patients at 6 different timepoints (including 12 months after ASCT) with Data-Independent Acquisition (DIA). As spectral library, all samples were grouped (ULC-OM vs NON-OM) and analyzed with Data Dependent Analysis (DDA). PCA plots and a volcano plot were generated in RStudio and differently regulated proteins were analyzed using GO analysis with g:Profiler.ResultsA different clustering of ULC-OM pools was found at baseline, weeks 2 and 3 after ASCT with TMT-labelled analysis. Using label-free analysis, week 1-3 samples clustered distinctly from the other timepoints. Unique and up-regulated proteins in the NON-OM group (DDA analysis) were involved in immune system-related processes, while those proteins in the ULC-OM group were intracellular proteins indicating cell lysis.ConclusionsThe salivary proteome in ASCT recipients has a tissue protective or tissue-damage signature, that corresponded with the absence or presence of ulcerative oral mucositis, respectively.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Oral mucositis
- Saliva
- TMT-labelled proteomics
- Label-free
- quantification
- Autologous hematopoietic stem cell transplantation
- Multiple myeloma
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
van Leeuwen, S. ...
-
Proctor, G. B.
-
Staes, A.
-
Laheij, Amga
-
Potting, C. M. J ...
-
Brennan, M. T.
-
visa fler...
-
von Bültzingslöw ...
-
Rozema, F. R.
-
Hazenberg, M. D.
-
Blijlevens, N. M ...
-
Raber-Durlacher, ...
-
Huysmans, Mcdnjm
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Odontologi
- Artiklar i publikationen
-
Bmc Oral Health
- Av lärosätet
-
Göteborgs universitet