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Comparison of immun...
Abstract
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- Background: Antibody-based immunoassays have enabled quantification of very low concentrations of phosphorylated tau (p-tau) protein forms in cerebrospinal fluid (CSF), aiding in the diagnosis of AD. Mass spectrometry enables absolute quantification of multiple p-tau variants within a single run. The goal of this study was to compare the performance of mass spectrometry assessments of p-tau181, p-tau217 and p-tau231 with established immunoassay techniques. Methods: We measured p-tau181, p-tau217 and p-tau231 concentrations in CSF from 173 participants from the TRIAD cohort and 394 participants from the BioFINDER-2 cohort using both mass spectrometry and immunoassay methods. All subjects were clinically evaluated by dementia specialists and had amyloid-PET and tau-PET assessments. Bland–Altman analyses evaluated the agreement between immunoassay and mass spectrometry p-tau181, p-tau217 and p-tau231. P-tau associations with amyloid-PET and tau-PET uptake were also compared. Receiver Operating Characteristic (ROC) analyses compared the performance of mass spectrometry and immunoassays p-tau concentrations to identify amyloid-PET positivity. Results: Mass spectrometry and immunoassays of p-tau217 were highly comparable in terms of diagnostic performance, between-group effect sizes and associations with PET biomarkers. In contrast, p-tau181 and p-tau231 concentrations measured using antibody-free mass spectrometry had lower performance compared with immunoassays. Conclusions: Our results suggest that while similar overall, immunoassay-based p-tau biomarkers are slightly superior to antibody-free mass spectrometry-based p-tau biomarkers. Future work is needed to determine whether the potential to evaluate multiple biomarkers within a single run offsets the slightly lower performance of antibody-free mass spectrometry-based p-tau quantification.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
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Therriault, Jose ...
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Woo, Marcel S.
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Salvadó, Gemma
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Gobom, Johan
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Karikari, Thomas
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Janelidze, Shore ...
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Servaes, Stijn
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Rahmouni, Nesrin ...
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Tissot, Cécile
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Ashton, Nicholas ...
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Lessa Benedet, A ...
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Montoliu-Gaya, L ...
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Macedo, Arthur C ...
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Lussier, Firoza ...
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Stevenson, Jenna
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Vitali, Paolo
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Friese, Manuel A ...
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Massarweh, Gassa ...
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Soucy, Jean Paul
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Pascoal, Tharick ...
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Stomrud, Erik
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Palmqvist, Sebas ...
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Mattsson-Carlgre ...
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Gauthier, Serge
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Zetterberg, Henr ...
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Hansson, Oskar
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Blennow, Kaj, 19 ...
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Rosa-Neto, Pedro
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Göteborgs universitet
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