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Changes in ion-chan...
Changes in ion-channels in the dorsal root ganglion after exposure to autologous nucleus pulposus and TNF. A rat experimental study
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- Håkansson, Joakim, 1975 (författare)
- RISE,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Institutionen för kemi och molekylärbiologi,Department of Laboratory Medicine,Department of Chemistry and Molecular Biology,Metodik, textil och medicinteknik,University of Gothenburg, Sweden
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- Juhlin, Oskar (författare)
- University of Gothenburg, Sweden
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- Hovannisyan, Armen (författare)
- University of Gothenburg, Sweden
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- Rosendahl, Jennifer (författare)
- RISE,Metodik, textil och medicinteknik
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- Bogestål, Yalda (författare)
- RISE,Metodik, textil och medicinteknik
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- Olmarker, Kjell, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,University of Gothenburg, Sweden
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(creator_code:org_t)
- Reed Elsevier India Pvt. Ltd. 2024
- 2024
- Engelska.
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Ingår i: Journal of Orthopaedics. - : Reed Elsevier India Pvt. Ltd.. - 0972-978X .- 2589-9082. ; 47, s. 23-27
- Relaterad länk:
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Purpose: It is known that contact of nucleus pulposus with the dorsal root ganglion may induce changes in nerve conduction and pain behavior. It has also been suggested that the behavioristic changes are caused by changes in voltage-gated ion channels, which in turn have been upregulated by TNF. Such upregulations have previously been shown for NaV 1.8 and NaV 1.9. In this investigation, we expanded the number of studied ion channels after the application of nucleus pulposus or TNF. Methods: Following removal of the left L4-5 fact joint, a disc puncture was performed and the dorsal root ganglion was exposed to nucleus pulposus (n = 5) and TNF (n = 5). Operated rats without disc puncture served as sham (n = 5) and 5 non-operated (naïve) rats were included. After 24 h, the DRGs were harvested and analyzed by quantitative PCR on validated pre-spotted primer plates displaying genes for 90 voltage-gated ion channels. Results: It was evident that the changes in operated animals were separate from the naïve rats. It was also apparent that gene expression changes in rats with nucleus pulposus or TNF application showed similar trends and were also separated from sham-operated animals. Conclusion: The application of nucleus pulposus and TNF onto the DRG in rats induces comparable changes in gene expression of several ion channels. Since the changes induced by TNF and NP are similar, one might also suspect that TNF mediates the NP-induced changes. However, such a mechanism needs further investigation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Ion channels
- Low back pain
- Rats
- Sciatica
- Spine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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