Nonradioactive in situ hybridization on frozen sections and whole mounts.

• Nonradioactive in situ hybridization offers a unique opportunity to study gene expression on samples with preserved histological information. This method makes it possible to locate not only where in a tissue a particular gene is expressed, but in many cases also in which specific cell type it is active. Here, we describe our current protocols for in situ hybridization on frozen sections or whole mounts of mouse embryos. The protocols included describe synthesis of a digoxigenin-labeled probe, tissue handling, hybridization of the probe to the mRNA expressed in the sample and signal detection.

Nyckelord

Animals

Digoxigenin

metabolism

Embryo

cytology

Female

Frozen Sections

Gene Expression

Histological Techniques

In Situ Hybridization

methods

Mice

Molecular Probes

chemical synthesis

Nucleic Acid Hybridization

methods

Pregnancy

RNA Probes

chemical synthesis

RNA

Messenger

metabolism

Publikations- och innehållstyp

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art (ämneskategori)