Effect of rescuing donated immature human oocytes derived after FSH/hCG stimulation following in vitro culture with or without Follicular Fluid Meiosis Activating Sterol (FF-MAS)--an embryo chromosomal and morphological analysis

• PURPOSE: Studies in mice and humans have shown that Follicular Fluid - Meiosis Activating Sterol (FF-MAS) induces meiotic maturation of immature oocytes in vitro. A multicenter, prospectively randomised study evaluated chromosomal status of embryos from FSH/hCG primed human immature oocytes, cultured with or without FF-MAS. METHODS: Denuded immature oocytes (n=365) were randomly allocated into inert control, FF-MAS 5 microM or 20 microM. Seventy +/-2 hours after ICSI on matured oocytes, all cleaved embryos were fixed for fluorescence in situ hybridisation analysis. RESULTS: Only 15% of oocytes resulted in cleaved embryos. GV oocytes matured at significantly lower rates (14% and 7%) in the two FF-MAS groups compared to the inert control group (47%). High rates of chromosomal abnormalities were found in all groups. CONCLUSION: Immature oocytes showed poor development with high rates of embryo chromosomal abnormalities. Exposure to FF-MAS in the concentrations, duration and/or formulation used in this study did not improve the results.

Nyckelord

Animals

Cell Culture Techniques

Cells

Cultured

Cholestenes/*pharmacology

Chorionic Gonadotropin/pharmacology

*Chromosome Aberrations

Cytogenetic Analysis

Embryo/*cytology

Embryonic Development

Female

Fertilization in Vitro

Follicle Stimulating Hormone/pharmacology

Humans

Mice

*Oocyte Donation

Oocytes/*drug effects

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)