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Simultaneous quanti...
Simultaneous quantification and genotyping of hepatitis B virus for genotypes A to G by real-time PCR and two-step melting curve analysis.
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Liu, Wen-Chun (författare)
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Mizokami, Masashi (författare)
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Buti, Maria (författare)
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- Lindh, Magnus, 1960 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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Young, Kung-Chia (författare)
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Sun, Koun-Tem (författare)
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Chi, Yun-Chan (författare)
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Li, Hsi-Hsien (författare)
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Chang, Ting-Tsung (författare)
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(creator_code:org_t)
- 2006
- 2006
- Engelska.
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Ingår i: Journal of clinical microbiology. - 0095-1137. ; 44:12, s. 4491-7
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Both the viral titer and the genotype significantly determine clinical outcomes and responses to antiviral treatment in chronic hepatitis B virus (HBV) infection. A method was developed for large-scale A-to-G genotyping with simultaneous viral quantification. The assay was run on a LightCycler instrument using hybridization probes. The genotype was determined from the melting points of the probes in a two-step manner. Set 1 amplicons differentiated genotypes B, E, and F from A, C, D, and G and simultaneously quantified viremia by real-time PCR. Melting curve analysis using the set 2-1 amplicon or the set 2-2 amplicon reaction mixture was then used to differentiate these genotype groups into single genotypes. HBV DNA quantification was consistent with that of the Amplicor assay and linear in a range from 10(2) to 10(13) copies/ml. By comparison with the restriction fragment length polymorphism method, 92.3% of 441 samples were accurately genotyped by the current assay. The method should be useful for genotyping and quantification of HBV DNA in areas where all genotypes exist.
Nyckelord
- DNA Primers
- DNA
- Viral
- chemistry
- genetics
- Genotype
- Hepatitis B
- diagnosis
- virology
- Hepatitis B virus
- classification
- genetics
- isolation & purification
- Humans
- Polymerase Chain Reaction
- methods
- Sensitivity and Specificity
- Transition Temperature
- Viral Load
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Liu, Wen-Chun
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Mizokami, Masash ...
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Buti, Maria
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Lindh, Magnus, 1 ...
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Young, Kung-Chia
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Sun, Koun-Tem
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visa fler...
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Chi, Yun-Chan
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Li, Hsi-Hsien
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Chang, Ting-Tsun ...
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visa färre...
- Artiklar i publikationen
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Journal of clini ...
- Av lärosätet
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Göteborgs universitet