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Gi-mediated muscari...
Gi-mediated muscarinic adenylyl cyclase inhibition in timolol-treated stunned porcine myocardium.
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- Fu, Michael, 1963 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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Ilebekk, A (författare)
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- Xu, X (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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visa fler...
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Pergola, G (författare)
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Eriksson, P (författare)
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Kirkebøen, K A (författare)
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Aksnes, G (författare)
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Hoebeke, Johan (författare)
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- Hjalmarson, Åke, 1937 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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visa färre...
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Acta physiologica Scandinavica. - 0001-6772. ; 151:3, s. 291-9
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- The Gi-mediated muscarinic receptor-adenylyl cyclase system was examined in stunned myocardium induced by either three or five brief ischaemic periods after beta-adrenoceptor blockade by timolol (0.1 mg kg-1). The mid-left anterior descending coronary artery was occluded for 2, 10 and 2 min in four pigs, and for 2, 2, 5, 10 and 2 min in four other pigs. All the ischaemic periods were separated by 30 min of reperfusion and the biopsies were obtained 60 min after the last ischaemic period. Segment length function was measured in the ischaemic region and in the control region supplied by the left circumflex artery. In the two groups, the percentage systolic shortening was reduced equally, to 59 +/- 9 and 58 +/- 10% of control in the region subjected to ischaemia and only minimally in the control region. The biopsies from the stunned region from both groups showed: (1) no change in either the affinity for carbachol or the number of binding sites of the muscarinic receptors; (2) no alterations in messenger RNA encoding for the alpha subunit-2 of the inhibitory guanine nucleotide binding protein, as demonstrated by northern blot and solution hybridization; (3) no change in membrane-bound inhibitory guanine nucleotide binding protein, as shown by enzyme immunoassay utilizing a specific anti-peptide antibody, and (4) unchanged inhibition of stimulated adenylyl cyclase activity. These results suggest that there is an intact inhibitory guanine nucleotide binding protein-mediated muscarinic receptor adenylyl cyclase system in the stunned porcine myocardium.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- Adenylate Cyclase
- antagonists & inhibitors
- Animals
- Blood Pressure
- drug effects
- Blotting
- Northern
- Coronary Circulation
- drug effects
- Female
- GTP-Binding Proteins
- physiology
- Heart
- drug effects
- Male
- Myocardial Contraction
- drug effects
- Myocardial Ischemia
- physiopathology
- Myocardium
- enzymology
- RNA
- metabolism
- Receptors
- Muscarinic
- drug effects
- physiology
- Signal Transduction
- drug effects
- Stroke Volume
- drug effects
- Swine
- Timolol
- pharmacology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Fu, Michael, 196 ...
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Ilebekk, A
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Xu, X
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Pergola, G
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Eriksson, P
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Kirkebøen, K A
-
visa fler...
-
Aksnes, G
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Hoebeke, Johan
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Hjalmarson, Åke, ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Fysiologi
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Acta physiologic ...
- Av lärosätet
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Göteborgs universitet