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Immunocytochemical ...
Immunocytochemical localization of M2 muscarinic receptors in rat ventricles with anti-peptide antibodies.
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- Fu, Michael, 1963 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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Schulze, W (författare)
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Wolf, W P (författare)
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visa fler...
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- Hjalmarson, Åke, 1937 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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Hoebeke, Johan (författare)
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visa färre...
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. - 0022-1554. ; 42:3, s. 337-43
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- We produced antibodies against a synthetic peptide corresponding to amino acids 168-192 of the second extracellular loop of the M2 human muscarinic receptor in rabbits. In immunoblot, affinity-purified antibodies specifically recognized a major band of rat ventricular muscarinic receptor protein with a molecular weight of about 80 KD. This recognition could be blocked by pre-incubation with peptide. Moreover, with both light (LM) and electron microscopic (EM) immunocytochemistry techniques, muscarinic receptors were detected on sarcolemma and T-tubules of rat cardiomyocytes. In addition, immunoreactions were localized in membranes of capillaries. Likewise, these reactivities were abolished by pre-incubation with peptide. These results suggest that the antibodies against the second extracellular loop of human M2 muscarinic receptor could specifically recognize rat ventricular muscarinic receptor protein and could be a powerful tool to study the fate of this receptor under different pathological or physiological conditions.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Acetylcholine
- chemistry
- Amino Acid Sequence
- Animals
- Antibodies
- Fluorescent Antibody Technique
- Heart Ventricles
- chemistry
- ultrastructure
- Humans
- Immunoblotting
- Immunohistochemistry
- Microscopy
- Immunoelectron
- methods
- Molecular Sequence Data
- Peptides
- Rabbits
- Rats
- Receptors
- Muscarinic
- analysis
- chemistry
- immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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