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Attenuated glial re...
Attenuated glial reactions and photoreceptor degeneration after retinal detachment in mice deficient in glial fibrillary acidic protein and vimentin.
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Nakazawa, Toru (författare)
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Takeda, Masumi (författare)
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Lewis, Geoffrey P (författare)
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Cho, Kin-Sang (författare)
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Jiao, Jianwei (författare)
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- Wilhelmsson, Ulrika, 1970 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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Fisher, Steven K (författare)
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- Pekny, Milos, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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Chen, Dong F (författare)
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Miller, Joan W (författare)
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(creator_code:org_t)
- Association for Research in Vision and Ophthalmology (ARVO), 2007
- 2007
- Engelska.
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Ingår i: Investigative ophthalmology & visual science. - : Association for Research in Vision and Ophthalmology (ARVO). - 0146-0404 .- 1552-5783. ; 48:6, s. 2760-8
- Relaterad länk:
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https://europepmc.or...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- PURPOSE: To characterize the reactions of retinal glial cells (astrocytes and Müller cells) to retinal injury in mice that lack glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) and to determine the role of glial cells in retinal detachment (RD)-induced photoreceptor degeneration. METHODS: RD was induced by subretinal injection of sodium hyaluronate in adult wild-type (WT) and GFAP-/-Vim-/- mice. Astroglial reaction and subsequent monocyte recruitment were quantified by measuring extracellular signal-regulated kinase (Erk) and c-fos activation and the level of expression of chemokine monocyte chemoattractant protein (MCP)-1 and by counting monocytes/microglia in the detached retinas. Immunohistochemistry, immunoblotting, real-time quantitative polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) were used. RD-induced photoreceptor degeneration was assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) and measurement of outer nuclear layer (ONL) thickness. RESULTS: RD-induced reactive gliosis, characterized by GFAP and vimentin upregulation, Erk and c-fos activation, MCP-1 induction, and increased monocyte recruitment in WT mice. Absence of GFAP and vimentin effectively attenuated reactive responses of retinal glial cells and monocyte infiltration. As a result, detached retinas of GFAP-/-Vim-/- mice exhibited significantly reduced numbers of TUNEL-positive photoreceptor cells and increased ONL thickness compared with those of WT mice. CONCLUSIONS: The absence of GFAP and vimentin attenuates RD-induced reactive gliosis and, subsequently, limits photoreceptor degeneration. Results of this study indicate that reactive retinal glial cells contribute critically to retinal damage induced by RD and provide a new avenue for limiting photoreceptor degeneration associated with RD and other retinal diseases or damage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Animals
- Blotting
- Western
- Chemokine CCL2
- metabolism
- Disease Models
- Animal
- Enzyme-Linked Immunosorbent Assay
- Extracellular Signal-Regulated MAP Kinases
- metabolism
- Fluorescent Antibody Technique
- Indirect
- Glial Fibrillary Acidic Protein
- deficiency
- physiology
- Gliosis
- etiology
- metabolism
- prevention & control
- In Situ Nick-End Labeling
- Male
- Mice
- Mice
- Inbred C57BL
- Mice
- Knockout
- Microglia
- physiology
- Monocytes
- physiology
- Neuroglia
- physiology
- Photoreceptors
- Vertebrate
- pathology
- Proto-Oncogene Proteins c-fos
- metabolism
- Retina
- pathology
- Retinal Degeneration
- etiology
- metabolism
- prevention & control
- Retinal Detachment
- complications
- Reverse Transcriptase Polymerase Chain Reaction
- Vimentin
- deficiency
- physiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Nakazawa, Toru
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Takeda, Masumi
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Lewis, Geoffrey ...
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Cho, Kin-Sang
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Jiao, Jianwei
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Wilhelmsson, Ulr ...
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visa fler...
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Fisher, Steven K
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Pekny, Milos, 19 ...
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Chen, Dong F
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Miller, Joan W
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
- Artiklar i publikationen
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Investigative op ...
- Av lärosätet
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Göteborgs universitet