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Nuclear translocati...
Nuclear translocation of apoptosis-inducing factor after focal cerebral ischemia
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Plesnila, N. (författare)
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- Zhu, Changlian, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology
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Culmsee, C. (författare)
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visa fler...
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Groger, M. (författare)
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Moskowitz, M. A. (författare)
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- Blomgren, Klas, 1963 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institute of Physiology and Pharmacology, Dept of Physiology,Institute for the Health of Women and Children, Dept of Paediatrics
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visa färre...
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: J Cereb Blood Flow Metab. ; 24:4, s. 458-66
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Signaling cascades associated with apoptosis contribute to cell death after focal cerebral ischemia. Cytochrome c release from mitochondria and the subsequent activation of caspases 9 and 3 are critical steps. Recently, a novel mitochondrial protein, apoptosis-inducing factor (AIF), has been implicated in caspase-independent programmed cell death following its translocation to the nucleus. We, therefore, addressed the question whether AIF also plays a role in cell death after focal cerebral ischemia. We detected AIF relocation from mitochondria to nucleus in primary cultured rat neurons 4 and 8 hours after 4 hours of oxygen/glucose deprivation. In ischemic mouse brain, AIF was detected within the nucleus 1 hour after reperfusion after 45 minutes occlusion of the middle cerebral artery. AIF translocation preceded cell death, occurred before or at the time when cytochrome c was released from mitochondria, and was evident within cells showing apoptosis-related DNA fragmentation. From these findings, we infer that AIF may be involved in neuronal cell death after focal cerebral ischemia and that caspase-independent signaling pathways downstream of mitochondria may play a role in apoptotic-like cell death after experimental stroke.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- Animals
- *Apoptosis
- Apoptosis Inducing Factor
- Blotting
- Western
- Cell Count
- Cell Nucleus/*metabolism
- Cells
- Cultured
- Cerebral Cortex/cytology/metabolism
- Culture Media
- Disease Models
- Animal
- Flavoproteins/*metabolism
- Glucose/metabolism
- Hippocampus/cytology/metabolism
- Immunohistochemistry
- Ischemic Attack
- Transient/*metabolism
- Male
- Membrane Proteins/*metabolism
- Mice
- Mice
- Inbred C57BL
- Mitochondria/metabolism
- Neurons/cytology/metabolism
- Oxygen/metabolism
- Rats
- Rats
- Sprague-Dawley
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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