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Studies on glycolip...
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Diswall, Mette,1979Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
(författare)
Studies on glycolipid antigens in small intestine and pancreas from alpha1,3-galactosyltransferase knockout miniature swine.
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
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Ovid Technologies (Wolters Kluwer Health),2007
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LIBRIS-ID:oai:gup.ub.gu.se/81224
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https://gup.ub.gu.se/publication/81224URI
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https://doi.org/10.1097/01.tp.0000287599.46165.15DOI
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Ämneskategori:art swepub-publicationtype
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BACKGROUND: To avoid hyperacute rejection of xeno-organs, alpha1,3-galactosyltransferase knockout (GalT-KO) pigs have been produced. Galalpha1,3Gal determinant elimination may expose cryptic carbohydrate antigens and/or generate new antigens. This is the first biochemical study of carbohydrate antigens in GalT-KO pig organs. METHODS: Neutral and acidic glycolipids were isolated from small intestine and pancreas of two GalT-KO and one wild-type (WT) pig. Glycolipid immune reactivity was tested on thin-layer chromatograms. Small intestine neutral glycolipids were separated by high-performance liquid chromatography and selected fractions were analyzed by proton nuclear magnetic resonance spectroscopy. Total gangliosides were quantified on thin-layer chromatograms and in microtiter wells. RESULTS: Using Galalpha1,3nLc4 glycolipid reference, total Galalpha1,3Gal glycolipid antigens in the WT animal was estimated at about 30 microg (small intestine) and 3 microg (pancreas) per gram of dry tissue. Galalpha1,3Gal determinants were not detected in GalT-KO tissues at a detection limit of less than 0.25% (small intestine) and 0.5% (pancreas) of the WT tissues. Isoglobotriaosylceramide (iGb3) was absent but trace amounts of Fuc-iGb3 was found in both GalT-KO and WT pig small intestine. Blood group H type 2 core saccharide compounds were increased in GalT-KO pancreas. Total amount of gangliosides was decreased in GalT-KO tissues. The alpha1,3-galactosyltransferase acceptor, N-acetyllactosamine determinant, was not increased in GalT-KO tissues. Human serum antibodies reacted with WT organ Galalpha1,3Gal antigens and gangliosides, of which the ganglioside reactivity remained in GalT-KO tissues. CONCLUSIONS: Knockout of porcine alpha1,3-galactosyltransferase gene results in elimination of Galalpha1,3Gal-terminated glycolipid compounds. GalT-KO genetic modification did not produce new compensatory glycolipid compounds reactive with human serum antibodies.
Ämnesord och genrebeteckningar
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Animals
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Animals
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Genetically Modified
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Antibodies
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blood
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Antigens
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analysis
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Galactosyltransferases
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deficiency
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genetics
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Glycolipids
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analysis
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immunology
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Humans
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Intestine
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Small
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immunology
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Pancreas
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immunology
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Swine
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Swine
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Miniature
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ångström, Jonas,1950Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xangjo
(författare)
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Dor, Frank J M F
(författare)
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Schuurman, Henk-Jan
(författare)
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Rydberg, Lennart,1944Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine(Swepub:gu)xrydle
(författare)
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Breimer, Michael,1951Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xbremi
(författare)
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Göteborgs universitetInstitutionen för kliniska vetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Transplantation: Ovid Technologies (Wolters Kluwer Health)84:10, s. 1348-560041-1337
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