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Sökning: onr:"swepub:oai:gup.ub.gu.se/81853" > Protein crystals in...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004426naa a2200409 4500
001oai:gup.ub.gu.se/81853
003SwePub
008240528s2008 | |||||||||||000 ||eng|
0247 a https://gup.ub.gu.se/publication/818532 URI
0247 a https://doi.org/10.1371/journal.pone.00028942 DOI
040 a (SwePub)gu
041 a eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Franqueville, Laure4 aut
2451 0a Protein crystals in Adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis.
264 c 2008-08-06
264 1b Public Library of Science (PLoS),c 2008
520 a Intranuclear crystalline inclusions have been observed in the nucleus of epithelial cells infected with Adenovirus serotype 5 (Ad5) at late steps of the virus life cycle. Using immuno-electron microscopy and confocal microscopy of cells infected with various Ad5 recombinants modified in their penton base or fiber domains, we found that these inclusions represented crystals of penton capsomers, the heteromeric capsid protein formed of penton base and fiber subunits. The occurrence of protein crystals within the nucleus of infected cells required the integrity of the fiber knob and part of the shaft domain. In the knob domain, the region overlapping residues 489-492 in the FG loop was found to be essential for crystal formation. In the shaft, a large deletion of repeats 4 to 16 had no detrimental effect on crystal inclusions, whereas deletion of repeats 8 to 21 abolished crystal formation without altering the level of fiber protein expression. This suggested a crucial role of the five penultimate repeats in the crystallisation process. Chimeric pentons made of Ad5 penton base and fiber domains from different serotypes were analyzed with respect to crystal formation. No crystal was found when fiber consisted of shaft (S) from Ad5 and knob (K) from Ad3 (heterotypic S5-K3 fiber), but occurred with homotypic S3K3 fiber. However, less regular crystals were observed with homotypic S35-K35 fiber. TB5, a monoclonal antibody directed against the Ad5 fiber knob was found by immunofluorescence microscopy to react with high efficiency with the intranuclear protein crystals in situ. Data obtained with Ad fiber mutants indicated that the absence of crystalline inclusions correlated with a lower infectivity and/or lower yields of virus progeny, suggesting that the protein crystals might be involved in virion assembly. Thus, we propose that TB5 staining of Ad-infected 293 cells can be used as a prognostic assay for the viability and productivity of fiber-modified Ad5 vectors.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng
7001 a Henning, Petra,d 1974u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology4 aut0 (Swepub:gu)xhenpe
7001 a Magnusson, Maria K,d 1972u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology4 aut0 (Swepub:gu)xmmara
7001 a Vigne, Emmanuelle4 aut
7001 a Schoehn, Guy4 aut
7001 a Blair-Zajdel, Maria E4 aut
7001 a Habib, Nagy4 aut
7001 a Lindholm, Leif,d 1940u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology4 aut0 (Swepub:gu)xlilei
7001 a Blair, G Eric4 aut
7001 a Hong, Saw See4 aut
7001 a Boulanger, Pierre4 aut
7102 a Göteborgs universitetb Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi4 org
7730 t PLoS ONEd : Public Library of Science (PLoS)g 3:8q 3:8x 1932-6203
8564 u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0002894&type=printable
8564 8u https://gup.ub.gu.se/publication/81853
8564 8u https://doi.org/10.1371/journal.pone.0002894

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