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Tumor necrosis fact...
Tumor necrosis factor-alpha and -beta upregulate the levels of osteoprotegerin mRNA in human osteosarcoma MG-63 cells.
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Brändström, Helena (författare)
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Jonsson, K B (författare)
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- Vidal, Olle (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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Ljunghall, S (författare)
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- Ohlsson, Claes, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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Ljunggren, O (författare)
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(creator_code:org_t)
- Elsevier BV, 1998
- 1998
- Engelska.
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Ingår i: Biochemical and biophysical research communications. - : Elsevier BV. - 0006-291X. ; 248:3, s. 454-7
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Osteoprotegerin (OPG) is a recently cloned soluble member of the tumor necrosis factor receptor family. OPG has been shown to inhibit osteoclast recruitment by binding to OPG-ligand, an osteoclast differentiating factor on osteoblastic stromal cells, thereby blocking osteoclastogenesis. In this report we have examined the effect of tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta) on OPG mRNA levels in the human osteosarcoma cell line MG-63. We demonstrate that both TNF-alpha and TNF-beta dose- and time-dependently upregulate the mRNA levels of OPG. The effect is significant at and above 5 pM of TNF-alpha and 1 pM of TNF-beta. The stimulatory effect on OPG mRNA levels in MG-63 cells was detected after 2 hrs of incubation with TNF-alpha or TNF-beta. These data demonstrate that the expression of OPG in osteoblasts, with subsequent effects on osteoclastogenesis, is regulated by TNFs.
Nyckelord
- Bone Neoplasms
- Glycoproteins
- biosynthesis
- Humans
- Kinetics
- Lymphotoxin-alpha
- biosynthesis
- Osteoprotegerin
- Osteosarcoma
- Polymerase Chain Reaction
- RNA
- Messenger
- biosynthesis
- Receptors
- Cytoplasmic and Nuclear
- Receptors
- Tumor Necrosis Factor
- biosynthesis
- Time Factors
- Transcription
- Genetic
- drug effects
- Tumor Cells
- Cultured
- Tumor Necrosis Factor-alpha
- pharmacology
- Up-Regulation
- drug effects
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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