Sökning: onr:"swepub:oai:gup.ub.gu.se/96632" >
The myxoid liposarc...
The myxoid liposarcoma FUS-DDIT3 fusion oncoprotein deregulates NF-kappaB target genes by interaction with NFKBIZ.
-
- Göransson, Melker, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology
-
- Andersson, Mattias K, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology
-
Forni, C (författare)
-
visa fler...
-
- Ståhlberg, Anders, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
-
- Andersson, Carola, 1972 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology
-
- Olofsson, A. (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology
-
Mantovani, R (författare)
-
- Åman, Pierre, 1953 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology
-
visa färre...
-
(creator_code:org_t)
- 2008-10-13
- 2009
- Engelska.
-
Ingår i: Oncogene. - : Springer Science and Business Media LLC. - 1476-5594 .- 0950-9232. ; 28:2, s. 270-8
- Relaterad länk:
-
https://www.nature.c...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- FUS (also called TLS), EWSR1 and TAF15 (also called TAF2N) are related genes involved in tumor type-specific fusion oncogenes in human malignancies. The FUS-DDIT3 fusion oncogene results from a t(12;16)(q13;p11) chromosome translocation and has a causative role in the initiation of myxoid/round cell liposarcomas (MLS/RCLS). The FUS-DDIT3 protein induces increased expression of the CAAT/enhancer-binding protein (C/EBP) and nuclear factor-kappaB (NF-kappaB)-controlled gene IL8, and the N-terminal FUS part is required for this activation. Chromatin immunoprecipitation analysis showed that FUS-DDIT3 binds the IL8 promoter. Expression studies of the IL8 promoter harboring a C/EBP-NF-kappaB composite site pinpointed the importance of NF-kappaB for IL8 expression in FUS-DDIT3-expressing cells. We therefore probed for possible interaction of FUS-DDIT3 with members of the NF-kappaB family. The nuclear factor NFKBIZ colocalizes with FUS-DDIT3 in nuclear structures, and immunoprecipitation experiments showed that FUS-DDIT3 binds the C-terminal of NFKBIZ. We also report that additional NF-kappaB-controlled genes are upregulated at the mRNA level in FUS-DDIT3-expressing cell lines and they can be induced by NFKBIZ. Taken together, the results indicate that FUS-DDIT3 deregulates some NF-kappaB-controlled genes through interactions with NFKBIZ. Similar mechanisms may be a part of the transformation process in other tumor types carrying FUS, EWSR1 and TAF15 containing fusion oncogenes.
Nyckelord
- Acute-Phase Proteins
- biosynthesis
- genetics
- Binding Sites
- CCAAT-Enhancer-Binding Proteins
- physiology
- Cell Line
- Tumor
- metabolism
- Cell Nucleus
- metabolism
- ultrastructure
- Fibrosarcoma
- metabolism
- pathology
- Humans
- Interleukin-6
- biosynthesis
- genetics
- Interleukin-8
- biosynthesis
- genetics
- Lipocalins
- biosynthesis
- genetics
- Liposarcoma
- Myxoid
- genetics
- pathology
- NF-kappa B
- physiology
- Neoplasm Proteins
- genetics
- physiology
- Nuclear Proteins
- physiology
- Oncogene Proteins
- Fusion
- genetics
- physiology
- Promoter Regions
- Genetic
- genetics
- Protein Binding
- Protein Interaction Mapping
- Proto-Oncogene Proteins
- biosynthesis
- genetics
- RNA-Binding Protein FUS
- genetics
- physiology
- Transcription Factor CHOP
- genetics
- physiology
- Transcription
- Genetic
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
Oncogene
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas