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Microarrays based o...
Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes
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- Wingren, Christer (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Steinhauer, Cornelia (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Ingvarsson, Johan (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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visa fler...
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Persson, E (författare)
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- Larsson, Kristina (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Borrebaeck, Carl (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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visa färre...
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(creator_code:org_t)
- 2005
- 2005
- Engelska.
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Ingår i: Micro Total Analysis Systems 2004, Vol 2. - 0260-6291. - 9780854048960 ; :297, s. 264-266
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
Stäng
- Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover, highly complex proteomes could be analysed without impairing the specificity and sensitivity of the setups. This is a pre-requisite for the design of high-density antibody arrays applied in high-throughput proteomics.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- scFv
- antibody microarrays
- antibody chips
- sensitivity
- affinity-tag
Publikations- och innehållstyp
- kon (ämneskategori)
- ref (ämneskategori)
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