Sökning: onr:"swepub:oai:lup.lub.lu.se:139347e1-819b-4673-9dfa-1d85c7a889ec" >
The human TRPA1 int...
The human TRPA1 intrinsic cold and heat sensitivity involves separate channel structures beyond the N-ARD domain
-
- Moparthi, Lavanya (författare)
- Linköpings universitet,Linköping University,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten,Wallenberg Centre for Molecular Medicine
-
- Sinica, Viktor (författare)
- Academy of Sciences of the Czech Republic,Czech Acad Sci, Czech Republic
-
- Moparthi, Vamsi K (författare)
- Linköpings universitet,Linköping University,Kemi,Tekniska fakulteten
-
visa fler...
-
- Kreir, Mohamed (författare)
- Janssen Pharmaceuticals, US,Janssen Pharmaceut NV, Belgium
-
- Vignane, Thibaut (författare)
- Leibniz Institute For Analytical Sciences (ISAS),Leibniz Inst Analyt Wissensch ISAS eV, Germany
-
- Filipovic, Milos R (författare)
- Leibniz Institute For Analytical Sciences (ISAS),Leibniz Inst Analyt Wissensch ISAS eV, Germany
-
- Vlachova, Viktorie (författare)
- Academy of Sciences of the Czech Republic,Czech Acad Sci, Czech Republic
-
- Zygmunt, Peter M (författare)
- Lund University,Lunds universitet,Neurofarmakologi och smärta,Forskargrupper vid Lunds universitet,Neuropharmacology and pain,Lund University Research Groups,Lund Univ, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2022-10-17
- 2022
- Engelska.
-
Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 13
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://liu.diva-por... (primary) (Raw object)
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- TRP channels sense temperatures ranging from noxious cold to noxious heat. Whether specialized TRP thermosensor modules exist and how they control channel pore gating is unknown. We studied purified human TRPA1 (hTRPA1) truncated proteins to gain insight into the temperature gating of hTRPA1. In patch-clamp bilayer recordings, ∆1-688 hTRPA1, without the N-terminal ankyrin repeat domain (N-ARD), was more sensitive to cold and heat, whereas ∆1-854 hTRPA1, also lacking the S1-S4 voltage sensing-like domain (VSLD), gained sensitivity to cold but lost its heat sensitivity. In hTRPA1 intrinsic tryptophan fluorescence studies, cold and heat evoked rearrangement of VSLD and the C-terminus domain distal to the transmembrane pore domain S5-S6 (CTD). In whole-cell electrophysiology experiments, replacement of the CTD located cysteines 1021 and 1025 with alanine modulated hTRPA1 cold responses. It is proposed that hTRPA1 CTD harbors cold and heat sensitive domains allosterically coupled to the S5-S6 pore region and the VSLD, respectively.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Nyckelord
- Alanine
- Ankyrin Repeat
- Hot Temperature
- Humans
- TRPA1 Cation Channel/genetics
- Thermosensing
- Tryptophan
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas