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Primary and hTERT-T...
Primary and hTERT-Transduced Mesothelioma-Associated Fibroblasts but Not Primary or hTERT-Transduced Mesothelial Cells Stimulate Growth of Human Mesothelioma Cells
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- Ries, Alexander (författare)
- Medical University of Vienna
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- Slany, Astrid (författare)
- University of Vienna
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- Pirker, Christine (författare)
- Medical University of Vienna
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- Mader, Johanna C. (författare)
- University of Vienna
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- Mejri, Doris (författare)
- Medical University of Vienna
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- Mohr, Thomas (författare)
- University of Vienna,Medical University of Vienna
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- Schelch, Karin (författare)
- Medical University of Vienna
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- Flehberger, Daniela (författare)
- Medical University of Vienna
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- Maach, Nadine (författare)
- Medical University of Vienna
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- Hashim, Muhammad (författare)
- Medical University of Vienna
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- Hoda, Mir Alireza (författare)
- Medical University of Vienna
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- Dome, Balazs (författare)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups,National Korányi Institute for Tuberculosis and Pulmonology, Hungary,Semmelweis University,Medical University of Vienna
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- Krupitza, Georg (författare)
- Medical University of Vienna
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- Berger, Walter (författare)
- Medical University of Vienna
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- Gerner, Christopher (författare)
- University of Vienna
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- Holzmann, Klaus (författare)
- Medical University of Vienna
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- Grusch, Michael (författare)
- Medical University of Vienna
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(creator_code:org_t)
- 2023
- 2023
- Engelska.
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Ingår i: Cells. - 2073-4409. ; 12:15
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Pleural mesothelioma (PM) is an aggressive malignancy that develops in a unique tumor microenvironment (TME). However, cell models for studying the TME in PM are still limited. Here, we have generated and characterized novel human telomerase reverse transcriptase (hTERT)-transduced mesothelial cell and mesothelioma-associated fibroblast (Meso-CAF) models and investigated their impact on PM cell growth. Pleural mesothelial cells and Meso-CAFs were isolated from tissue of pneumothorax and PM patients, respectively. Stable expression of hTERT was induced by retroviral transduction. Primary and hTERT-transduced cells were compared with respect to doubling times, hTERT expression and activity levels, telomere lengths, proteomes, and the impact of conditioned media (CM) on PM cell growth. All transduced derivatives exhibited elevated hTERT expression and activity, and increased mean telomere lengths. Cell morphology remained unchanged, and the proteomes were similar to the corresponding primary cells. Of note, the CM of primary and hTERT-transduced Meso-CAFs stimulated PM cell growth to the same extent, while CM derived from mesothelial cells had no stimulating effect, irrespective of hTERT expression. In conclusion, all new hTERT-transduced cell models closely resemble their primary counterparts and, hence, represent valuable tools to investigate cellular interactions within the TME of PM.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- conditioned medium
- hTERT
- human telomerase reverse transcriptase
- mesothelioma-associated fibroblasts
- pleural mesothelial cells
- pleural mesothelioma
- tumor microenvironment
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
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Cells
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Till lärosätets databas
- Av författaren/redakt...
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Ries, Alexander
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Slany, Astrid
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Pirker, Christin ...
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Mader, Johanna C ...
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Mejri, Doris
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Mohr, Thomas
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visa fler...
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Schelch, Karin
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Flehberger, Dani ...
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Maach, Nadine
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Hashim, Muhammad
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Hoda, Mir Alirez ...
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Dome, Balazs
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Krupitza, Georg
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Berger, Walter
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Gerner, Christop ...
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Holzmann, Klaus
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Grusch, Michael
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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Cells
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Lunds universitet