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Extrapancreatic try...
Extrapancreatic trypsin-2 cleaves proteinase-activated receptor-2.
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Alm, Anna-Karin (författare)
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- Gagnemo Persson, Rebecca (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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- Sorsa, Timo (författare)
- University of Helsinki
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Sundelin, Johan (författare)
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(creator_code:org_t)
- Elsevier BV, 2000
- 2000
- Engelska 6 s.
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Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 1090-2104 .- 0006-291X. ; 275:1, s. 77-83
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Proteinase-activated receptors (PARs) are activated by proteolytic removal of a short amino terminal peptide, thus exposing a new amino terminus that functions as a tethered ligand that activates the receptor. With the aim to identify and study potential activators of PAR-2 we have developed a new method to measure proteolytic cleavage of PARs. PAR-2 was tagged with the insulin C-peptide that upon receptor cleavage is released and quantified using an ELISA. The modified receptor, shown to be functional in mouse 3T3 cells, was expressed in an insect cell line and the ability of different proteinases to cleave PAR-2 was studied. Two different mast cell tryptases cleaved PAR-2 in a concentration dependent manner, but were much less potent than pancreatic trypsin and trypsin-2 isolated from a carcinoma cell line. Pancreatic trypsin and trypsin-2 were almost equally effective at cleaving PAR-2 suggesting that extrapancreatic trypsins are potential in vivo activators of PAR-2.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- proteinase-activated receptor-2; mast cell tryptase; trypsin-2
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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