Sökning: onr:"swepub:oai:lup.lub.lu.se:176da897-f7ea-466a-bc82-be05260d4bc6" >
N-acetylation of ar...
N-acetylation of arylalkylmines (serotonin and tryptamine) in the crayfish brain.
-
- Dubbels, R (författare)
- University of Bremen
-
- Elofsson, Rolf (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science
-
(creator_code:org_t)
- Elsevier BV, 1989
- 1989
- Engelska.
-
Ingår i: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. - : Elsevier BV. - 1532-0456 .- 0306-4492. ; 93, s. 307-312
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- 1. The presence of N-acetyltransferase in the crayfish brain (Pacifastacus leniusculus) was investigated. The arylalkylamines tryptamine and 14C-serotonin, are acetylated by crayfish brain homogenates with the acetyl donors 3H-acetyl coenzyme A and N, S-diacetylcysteamine, respectively.2. Resolution of serotonin metabolites by thin layer chromatography (chloroform/methanol/glacial acetic acid, 93 + 7 + 1 and ethyl acetate) revealed only one radioactive product (N-acetylserotonin, Rf 0.31) after enzymatic reaction. For example, melatonin and 5-hydroxyindole acetic acid could not be detected.3. This was also confirmed by reversed phase high performance liquid chromatography indicating the absence of monoamine oxidase and hydroxyindole-O-methyltransferase activity under the experimental conditions used.4. Inhibitory studies with cystamine suggest the involvement of heat sensitive disulfide compounds in the regulation of crayfish brain-N-acetyltransferase.5. N-acetylation seems to be a major pathway in the metabolism of biogenic amines in the crayfish central nervous system.
Ämnesord
- NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas