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Rapid Identificatio...
Rapid Identification of the Tumor-Specific Reactive TIL Repertoire via Combined Detection of CD137, TNF, and IFNγ, Following Recognition of Autologous Tumor-Antigens
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- Draghi, Arianna (författare)
- Copenhagen University Hospital
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- Chamberlain, Christopher Aled (författare)
- Copenhagen University Hospital
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- Khan, Shawez (författare)
- Copenhagen University Hospital
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- Papp, Krisztian (författare)
- Eötvös Loránd University
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- Lauss, Martin (författare)
- Lund University,Lunds universitet,Melanoma Genomics,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Soraggi, Samuele (författare)
- Aarhus University
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- Radic, Haja Dominike (författare)
- Copenhagen University Hospital
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- Presti, Mario (författare)
- Copenhagen University Hospital
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- Harbst, Katja (författare)
- Lund University,Lunds universitet,Lunds Melanomstudiegrupp,Forskargrupper vid Lunds universitet,Melanoma Genomics,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund Melanoma Study Group,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Gokuldass, Aishwarya (författare)
- Copenhagen University Hospital
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- Kverneland, Anders (författare)
- Copenhagen University Hospital
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- Nielsen, Morten (författare)
- Copenhagen University Hospital
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- Westergaard, Marie Christine Wulff (författare)
- Copenhagen University Hospital
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- Andersen, Mads Hald (författare)
- Copenhagen University Hospital
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- Csabai, Istvan (författare)
- Eötvös Loránd University
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- Jönsson, Göran (författare)
- Lund University,Lunds universitet,Lunds Melanomstudiegrupp,Forskargrupper vid Lunds universitet,Melanoma Genomics,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Melanom,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund Melanoma Study Group,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Melanoma,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Szallasi, Zoltan (författare)
- Danish Cancer Society
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- Svane, Inge Marie (författare)
- Copenhagen University Hospital
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- Donia, Marco (författare)
- Copenhagen University Hospital
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(creator_code:org_t)
- 2021-10-11
- 2021
- Engelska.
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Ingår i: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 12
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://www.frontier...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Detecting the entire repertoire of tumor-specific reactive tumor-infiltrating lymphocytes (TILs) is essential for investigating their immunological functions in the tumor microenvironment. Current in vitro assays identifying tumor-specific functional activation measure the upregulation of surface molecules, de novo production of antitumor cytokines, or mobilization of cytotoxic granules following recognition of tumor-antigens, yet there is no widely adopted standard method. Here we established an enhanced, yet simple, method for identifying simultaneously CD8+ and CD4+ tumor-specific reactive TILs in vitro, using a combination of widely known and available flow cytometry assays. By combining the detection of intracellular CD137 and de novo production of TNF and IFNγ after recognition of naturally-presented tumor antigens, we demonstrate that a larger fraction of tumor-specific and reactive CD8+ TILs can be detected in vitro compared to commonly used assays. This assay revealed multiple polyfunctionality-based clusters of both CD4+ and CD8+ tumor-specific reactive TILs. In situ, the combined detection of TNFRSF9, TNF, and IFNG identified most of the tumor-specific reactive TIL repertoire. In conclusion, we describe a straightforward method for efficient identification of the tumor-specific reactive TIL repertoire in vitro, which can be rapidly adopted in most cancer immunology laboratories.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- CD137 (4-1BB)
- immune-monitoring
- immune-responses to cancer
- single-cell technologies
- tumor-infiltrating lymphocytes (TILs)
- tumor-specific activation
- tumor-specific reactivity
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Draghi, Arianna
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Chamberlain, Chr ...
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Khan, Shawez
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Papp, Krisztian
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Lauss, Martin
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Soraggi, Samuele
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visa fler...
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Radic, Haja Domi ...
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Presti, Mario
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Harbst, Katja
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Gokuldass, Aishw ...
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Kverneland, Ande ...
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Nielsen, Morten
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Westergaard, Mar ...
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Andersen, Mads H ...
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Csabai, Istvan
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Jönsson, Göran
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Szallasi, Zoltan
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Svane, Inge Mari ...
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Donia, Marco
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Frontiers in Imm ...
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Lunds universitet