Bioprospecting Microbial Diversity for Lignin Valorization : Dry and Wet Screening Methods

Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.

Bruce, ThiagoCatholic University Of Brasilia (UCB) (författare)
Silva, Caio de Oliveira GorgulhoUniversity of Brasilia (författare)
Fillho, Edivaldo Ximenes FerreiraUniversity of Brasilia (författare)
Noronha, Eliane FerreiraUniversity of Brasilia (författare)
Carlquist, MagnusLund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)tmb-mca (författare)
Parachin, Nádia SkorupaCatholic University Of Brasilia (UCB)(Swepub:lu)tmb-nsn (författare)
Catholic University Of Brasilia (UCB)University of Brasilia (creator_code:org_t)

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