Sökning: onr:"swepub:oai:lup.lub.lu.se:24dfdeda-5006-482b-ba55-0d120dd89f7c" > Deep learning-enhan...
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000 | 03477naa a2200409 4500 | |
001 | oai:lup.lub.lu.se:24dfdeda-5006-482b-ba55-0d120dd89f7c | |
003 | SwePub | |
008 | 210514s2021 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/24dfdeda-5006-482b-ba55-0d120dd89f7c2 URI |
024 | 7 | a https://doi.org/10.1038/s41592-021-01136-02 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Wagner, Nilsu European Molecular Biology Laboratory Heidelberg4 aut |
245 | 1 0 | a Deep learning-enhanced light-field imaging with continuous validation |
264 | c 2021-05-07 | |
264 | 1 | b Springer Science and Business Media LLC,c 2021 |
520 | a Visualizing dynamic processes over large, three-dimensional fields of view at high speed is essential for many applications in the life sciences. Light-field microscopy (LFM) has emerged as a tool for fast volumetric image acquisition, but its effective throughput and widespread use in biology has been hampered by a computationally demanding and artifact-prone image reconstruction process. Here, we present a framework for artificial intelligence-enhanced microscopy, integrating a hybrid light-field light-sheet microscope and deep learning-based volume reconstruction. In our approach, concomitantly acquired, high-resolution two-dimensional light-sheet images continuously serve as training data and validation for the convolutional neural network reconstructing the raw LFM data during extended volumetric time-lapse imaging experiments. Our network delivers high-quality three-dimensional reconstructions at video-rate throughput, which can be further refined based on the high-resolution light-sheet images. We demonstrate the capabilities of our approach by imaging medaka heart dynamics and zebrafish neural activity with volumetric imaging rates up to 100 Hz. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Radiologi och bildbehandling0 (SwePub)302082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Radiology, Nuclear Medicine and Medical Imaging0 (SwePub)302082 hsv//eng |
700 | 1 | a Beuttenmueller, Fynnu Heidelberg University4 aut |
700 | 1 | a Norlin, Nilsu Lund University,Lunds universitet,Molekylär neuromodulering,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Medicinska fakulteten,Molecular Neuromodulation,Lund University Research Groups,Faculty of Medicine,European Molecular Biology Laboratory Heidelberg4 aut0 (Swepub:lu)ni5154no |
700 | 1 | a Gierten, Jakobu University Hospital Heidelberg4 aut |
700 | 1 | a Boffi, Juan Carlos4 aut |
700 | 1 | a Wittbrodt, Joachimu Heidelberg University4 aut |
700 | 1 | a Weigert, Martinu Swiss Federal Institute of Technology4 aut |
700 | 1 | a Hufnagel, Lars4 aut |
700 | 1 | a Prevedel, Robertu European Molecular Biology Laboratory (EMBL Rome)4 aut |
700 | 1 | a Kreshuk, Anna4 aut |
710 | 2 | a European Molecular Biology Laboratory Heidelbergb Heidelberg University4 org |
773 | 0 | t Nature Methodsd : Springer Science and Business Media LLCg 18:5, s. 557-563q 18:5<557-563x 1548-7105x 1548-7091 |
856 | 4 | u http://dx.doi.org/10.1038/s41592-021-01136-0y FULLTEXT |
856 | 4 | u https://doi.org/10.1101/2020.07.30.228924 |
856 | 4 8 | u https://lup.lub.lu.se/record/24dfdeda-5006-482b-ba55-0d120dd89f7c |
856 | 4 8 | u https://doi.org/10.1038/s41592-021-01136-0 |
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