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Ultrasonic-trap-enh...
Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.
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Wiklund, M (författare)
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- Spégel, Peter (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Staffan (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Hertz, H M (författare)
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: Ultrasonics. - 0041-624X. ; 41:4, s. 329-333
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Ultrasonic trapping
- Capillary electrophoresis
- Particle separation
- Concentration limit of detection
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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