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Affinity screening ...
Affinity screening for weak monoclonal antibodies
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- Leickt, Lisa (författare)
- University of Kalmar
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- Grubb, Anders (författare)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cystatin C, njursjukdom, amyloidos och antibiotika,Forskargrupper vid Lunds universitet,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Cystatin C, renal disease, amyloidosis and antibiotics,Lund University Research Groups,Skåne University Hospital
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- Ohlson, Sten (författare)
- University of Kalmar
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(creator_code:org_t)
- Elsevier BV, 1998
- 1998
- Engelska.
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Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 220:1-2, s. 19-24
- Relaterad länk:
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http://dx.doi.org/10...
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Abstract
Ämnesord
Stäng
- When selecting for monoclonal antibodies of a desired affinity, affinity chromatography can be a feasible alternative. This is of particular interest when low affinity monoclonal antibodies (dissociation constant (Kd) > 10(-4) M) are screened, as they are not easily recognised by traditional immunoassay procedures. In this study we have evaluated this approach by monitoring low affinity monoclonal antibodies on high performance liquid affinity chromatography columns with oligosaccharides, dinitrophenol and digoxin as immobilised antigen. Crude monoclonal antibodies in ascites or cell culture supematants, directed against these antigens, were retarded or adsorbed according to affinity or avidity on the antigen columns. Based on antibody retention, we were able to select hybridomas with the desired low affinity characteristics.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Animals
- Antibodies, Monoclonal/immunology
- Antibody Affinity
- Ascitic Fluid/immunology
- Chromatography, Affinity
- Chromatography, High Pressure Liquid
- Digoxin/immunology
- Dinitrophenols/immunology
- Hemocyanins/immunology
- Immunoglobulin M/immunology
- Mice
- Oligosaccharides/immunology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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