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Prostate-specific a...
Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin
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- Lilja, H (författare)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
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- Christensson, A (författare)
- Skåne University Hospital
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Dahlén, U (författare)
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Matikainen, M T (författare)
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Nilsson, O (författare)
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Pettersson, K (författare)
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Lövgren, T (författare)
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(creator_code:org_t)
- 1991-09-01
- 1991
- Engelska 8 s.
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Ingår i: Clinical Chemistry. - : Oxford University Press (OUP). - 0009-9147 .- 1530-8561. ; 37:9, s. 25-1618
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Nyckelord
- Animals
- Antibodies, Monoclonal
- Antigens, Neoplasm/blood
- Biomarkers, Tumor/blood
- Chromatography, Gel
- Electrophoresis, Agar Gel
- Electrophoresis, Polyacrylamide Gel
- Epitopes/analysis
- Female
- Humans
- Male
- Mice
- Mice, Inbred BALB C
- Prostate-Specific Antigen
- alpha 1-Antichymotrypsin/immunology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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