Sökning: onr:"swepub:oai:lup.lub.lu.se:32ae4433-593f-4fce-b497-44204f0fcb25" > Development of a Mo...
Fältnamn | Indikatorer | Metadata |
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000 | 04314naa a2200421 4500 | |
001 | oai:lup.lub.lu.se:32ae4433-593f-4fce-b497-44204f0fcb25 | |
003 | SwePub | |
008 | 201215s2020 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/32ae4433-593f-4fce-b497-44204f0fcb252 URI |
024 | 7 | a https://doi.org/10.3389/fcimb.2020.5715782 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Andersson, Tildeu Lund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)ti3042an |
245 | 1 0 | a Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum |
264 | c 2020-11-20 | |
264 | 1 | b Frontiers Media SA,c 2020 |
520 | a Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (KD = 2.75 x 10-7 M) and demonstrated a statistically significant difference (p < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng |
653 | a alpha hemolysin | |
653 | a molecular imprinting | |
653 | a sepsis | |
653 | a SPR | |
653 | a Staphylococcus aureus | |
700 | 1 | a Bläckberg, Annau Lund University,Lunds universitet,Translationell infektionsmedicin,Forskargrupper vid Lunds universitet,Translational infection medicine,Lund University Research Groups,Skåne University Hospital4 aut0 (Swepub:lu)an7442bl |
700 | 1 | a Lood, Rolfu Lund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)med-rll |
700 | 1 | a Ertürk Bergdahl, Gizemu Lund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)gi2856er |
710 | 2 | a Experimental Infection Medicineb Forskargrupper vid Lunds universitet4 org |
773 | 0 | t Frontiers in cellular and infection microbiologyd : Frontiers Media SAg 10q 10x 2235-2988 |
856 | 4 | u http://dx.doi.org/10.3389/fcimb.2020.571578x freey FULLTEXT |
856 | 4 | u https://www.frontiersin.org/articles/10.3389/fcimb.2020.571578/pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/32ae4433-593f-4fce-b497-44204f0fcb25 |
856 | 4 8 | u https://doi.org/10.3389/fcimb.2020.571578 |
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