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CCD-camera based ca...
CCD-camera based capillary chemiluminescent detection of retinol binding protein
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- Momeni, Naghi (författare)
- TMS CHEM AB
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- Ramanathan, Kumaran (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Larsson, P-O. (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Danielsson, Bengt (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Bengmark, Stig (författare)
- Ideon AB
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- Khayyami, Masoud (författare)
- TMS CHEM AB
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(creator_code:org_t)
- 1999
- 1999
- Engelska 7 s.
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Ingår i: Analytica Chimica Acta. - 0003-2670. ; 387:1, s. 21-27
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP determination was between 11 pg ml−1 and 11 ng ml−1 with a coefficient of variation of 5–11% (n=6) over this range.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Nyckelord
- Chemiluminescence assay
- glass capillaries
- Horse radish peroxidase
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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