Sökning: onr:"swepub:oai:lup.lub.lu.se:41a841f9-4a61-4865-b972-5df99b8e9743" > Diagnosis of acute ...
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000 | 03281naa a2200421 4500 | |
001 | oai:lup.lub.lu.se:41a841f9-4a61-4865-b972-5df99b8e9743 | |
003 | SwePub | |
008 | 160401s1992 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/11069202 URI |
024 | 7 | a https://doi.org/10.1111/j.1365-2141.1992.tb06463.x2 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Borrow, Julian4 aut |
245 | 1 0 | a Diagnosis of acute promyelocytic leukaemia by RT-PCR: detection of PML-RARA and RARA-PML fusion transcripts |
264 | 1 | b Wiley,c 1992 |
520 | a Acute promyelocytic leukaemia (APL; AML M3) is identified by a unique t(15;17) translocation which fuses the PML gene to the retinoic acid receptor alpha gene (RARA). Reverse transcription coupled with the polymerase chain reaction (RT-PCR) has been used to develop a diagnostic test for APL based on the PML-RARA fusion message. Separate PCR assays were designed to amplify either PML-RARA (15q+ derived) or RARA-PML (17q- derived) chimaeric transcripts. PML-RARA transcripts were detected in every case from a series of 18 APL patients with cytogenetically confirmed t(15;17) translocations, whereas RARA-PML messages were detected in only 67% (12/18) of these patients. This suggests that it is the 15q+ derivative which mediates leukaemogenesis. Furthermore the PCR approach (or Southern analysis) may be used to identify in which of the alternative PML introns the breakpoint occurs; 52% of cases (15/29 patients) utilize a 5' PML intron and 48% the 3' intron (14/29 cases). Neither the choice of PML intron nor the expression of the 17q- derivative could be correlated with the microgranular variant of APL (M3V), overall survival rate, age, sex or presence of coagulopathy. Finally, the fusion message is undetectable in five remission samples. This indicates a possible use for RT-PCR in monitoring remission patients for evidence of relapse. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Hematologi0 (SwePub)302022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Hematology0 (SwePub)302022 hsv//eng |
700 | 1 | a Goddard, Audrey D4 aut |
700 | 1 | a Gibbons, Barbara4 aut |
700 | 1 | a Katz, Fay4 aut |
700 | 1 | a Swirsky, David4 aut |
700 | 1 | a Fioretos, Thoasu Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)kgen-tfi |
700 | 1 | a Dube, Ian4 aut |
700 | 1 | a Winfield, David A4 aut |
700 | 1 | a Kingston, Judith4 aut |
700 | 1 | a Hagemeijer, Anne4 aut |
700 | 1 | a Rees, John K H4 aut |
700 | 1 | a Lister, T Andrew4 aut |
700 | 1 | a Solomon, Ellen4 aut |
710 | 2 | a Avdelningen för klinisk genetikb Institutionen för laboratoriemedicin4 org |
773 | 0 | t British Journal of Haematologyd : Wileyg 82:3, s. 529-540q 82:3<529-540x 0007-1048x 1365-2141 |
856 | 4 | u http://dx.doi.org/10.1111/j.1365-2141.1992.tb06463.xy FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/1106920 |
856 | 4 8 | u https://doi.org/10.1111/j.1365-2141.1992.tb06463.x |
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