onr:"swepub:oai:lup.lub.lu.se:43d0fb22-7f82-4384-87e4-8926e7393f62"
Sökning: onr:"swepub:oai:lup.lub.lu.se:43d0fb22-7f82-4384-87e4-8926e7393f62" > Direct chromatograp...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04234naa a2200337 4500 | |
| 001 | oai:lup.lub.lu.se:43d0fb22-7f82-4384-87e4-8926e7393f62 | |
| 003 | SwePub | |
| 008 | 160401s2003 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/1290512 URI |
| 024 | 7 | a https://doi.org/10.1016/S0021-9673(02)01871-X2 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Arvidsson, Päru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-par |
| 245 | 1 0 | a Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent |
| 264 | 1 | c 2003 |
| 520 | a A continuous supermacroporous matrix has been developed allowing direct capture of enzyme from non-clarified crude cell homogenate at high flow-rates. The continuous supermacroporous matrix has been produced by radical co-polymerization of acrylamide, allyl glycidyl ether and N,N′-methylene-bis(acrylamide) which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the column contains a continuous matrix having interconnected pores of 10–100 m size. Iminodiacetic acid covalently coupled to the cryogel is a rendering possibility for immobilized metal affinity chromatographic purification of recombinant His-tagged lactate dehydrogenase, (His)6-LDH, originating from thermophilic bacterium Bacillus stearothermophilus, but expressed in Escherichia coli. The large pore size of the adsorbent makes it possible to process particulate-containing material without blocking the column. No preliminary filtration or centrifugation is needed before application of crude extract on the supermacroporous column. A total of 210 ml crude homogenate, 75 ml of it non-clarified, was processed on a single 5.0 ml supermacroporous column at flow speeds up to 12.5 ml/min without noticeable impairment of the column properties. Mechanically the cryogel adsorbent is very stable. The continuous matrix could easily be removed from the column, dried at 70 °C and kept in a dry state. After rehydration and reinsertion of the matrix into an empty column, (His)6-LDH was purified as efficiently as on the newly prepared column. The procedure of manufacturing the supermacroporous continuous cryogel is technically simple. Starting materials and initiators are cheap and available and are simply mixed and frozen under specified conditions. Altogether these qualities reveal that the supermacroporous continuous cryogels is a very interesting alternative to existing methods of protein purification from particulate-containing crude extracts | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
| 653 | a Enzymes | |
| 700 | 1 | a Plieva, F4 aut |
| 700 | 1 | a Lozinsky, V4 aut |
| 700 | 1 | a Galaev, Igoru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-iga |
| 700 | 1 | a Mattiasson, Bou Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-bma |
| 710 | 2 | a Bioteknikb Centrum för tillämpade biovetenskaper4 org |
| 773 | 0 | t Journal of Chromatography Ag 986:2, s. 275-290q 986:2<275-290x 0021-9673 |
| 856 | 4 | u http://dx.doi.org/10.1016/S0021-9673(02)01871-Xy FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/129051 |
| 856 | 4 8 | u https://doi.org/10.1016/S0021-9673(02)01871-X |
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