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Component of the al...
Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondria
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- Rasmusson, Allan G. (författare)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
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Møller, Ian M. (författare)
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- Palmer, John M. (författare)
- Imperial College London
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(creator_code:org_t)
- 1990
- 1990
- Engelska 4 s.
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Ingår i: FEBS Letters. - 0014-5793. ; 259:2, s. 311-314
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- (Arum maculatum, Plant, Mitochondria)
- Alternative pathway
- Inside-out submitochondrial particle
- Mitoplast
- Trypsin
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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