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Precision and sensi...
Precision and sensitivity of the measurement of 15 N enrichment in D-alanine from bacterial cell walls using positive/negative ion mass spectrometry
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- Tunlid, Anders (författare)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science
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- Odham, Göran (författare)
- Lund University
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- Findlay, Robert H. (författare)
- Florida State University
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- White, David C. (författare)
- Florida State University
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(creator_code:org_t)
- 1985
- 1985
- Engelska.
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Ingår i: Journal of Microbiological Methods. - 0167-7012. ; 3:3-4, s. 237-245
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Sensitive detection of cellular components from specific groups of microbes can be utilized as 'signatures' in the examination of microbial consortia from soils, sediments or biofilms. Utilizing capillary gas chromatography/mass spectrometry and stereospecific derivatizing agents, D-alanine, a component localized in the prokaryotic (bacteria) cell wall, can be detected reproducibly. Enrichments of D- 15 N]alanine determined in E. coli grown with [ 15 N]ammonia can be determined with precision at 1.0 atom%. Chemical ionization with methane gas and the detection of negative ions (M-HF) - · and (M-F or M+H - HF) - formed from the heptafluorobutyryl D-2 butanol ester of D-alanine allowed as little as 8 pg (90 fmol) to be detected reproducibly. This method can be utilized to define the metabolic activity in terms of 15 N incorporation at the level of 10 3 -10 4 cells, as a function of the 15 N- 14 N ratio.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Bacterial cell wall
- Capillary gas chromatography/mass spectrometry
- D-Alanine
- Positive/negative ion chemical ionization
- Selected ion monitoring
- Stable isotope labeling
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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