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The mitochondrial L...
The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis
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- Li, Ying (författare)
- University of Western Australia, Perth
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- Belt, Katharina (författare)
- University of Western Australia, Perth
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- Alqahtani, Saad F. (författare)
- University of Western Australia, Perth
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- Saha, Saurabh (författare)
- University of Western Australia, Perth
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- Fenske, Ricarda (författare)
- University of Western Australia, Perth
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- Van Aken, Olivier (författare)
- Lund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,University of Western Australia, Perth
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- Whelan, James (författare)
- La Trobe University
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- Millar, A. Harvey (författare)
- University of Western Australia, Perth
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- Murcha, Monika W. (författare)
- University of Western Australia, Perth
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- Huang, Shaobai (författare)
- University of Western Australia, Perth
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(creator_code:org_t)
- 2022-02-26
- 2022
- Engelska 14 s.
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Ingår i: Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 110:2, s. 499-512
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- assembly factors
- complex II
- iron–sulfur cluster insertion
- mitochondrial metabolism
- succinate dehydrogenase
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Li, Ying
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Belt, Katharina
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Alqahtani, Saad ...
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Saha, Saurabh
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Fenske, Ricarda
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Van Aken, Olivie ...
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visa fler...
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Whelan, James
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Millar, A. Harve ...
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Murcha, Monika W ...
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Huang, Shaobai
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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Plant Journal
- Av lärosätet
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Lunds universitet