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Detection of vitami...
Detection of vitamin K-dependent proteins in venoms with a monoclonal antibody specific for gamma-carboxyglutamic acid.
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- Brown, Mark A. (författare)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
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Hambe, B (författare)
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Furie, B (författare)
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Furie, B C (författare)
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- Stenflo, Johan (författare)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
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- Stenberg, Leisa (författare)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
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(creator_code:org_t)
- 2002
- 2002
- Engelska.
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Ingår i: Toxicon. - 0041-0101. ; 40:4, s. 447-453
- Relaterad länk:
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http://www.ncbi.nlm....
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visa fler...
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and immunofluorescence assays. Amino acid analyses were also performed on most samples. A survey of 21 snake species from 12 genera detected gamma-carboxylated polypeptides only in venom of snakes from the elapid subfamily Acanthophiinae. Gla-containing polypeptides were also observed in cone snail venom but not in venom or toxic salivary secretions from several other organisms. The Gla-specific antibody used here provides a simple immunochemical means to detect gamma-carboxylated polypeptides in venom and may allow new species to be identified that utilize Gla in the biosynthesis of toxic polypeptides.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Nyckelord
- Biological Assay : methods
- Anura
- Antibodies Monoclonal : diagnostic use
- Blood Proteins : metabolism
- Elapid Venoms : chemistry
- Blotting Western
- Factor Xa : analysis : metabolism
- Fluorescent Antibody Technique
- Mollusk Venoms : chemistry
- Peptides : analysis : chemistry
- Salivary Glands : chemistry
- Support Non-U.S. Gov't
- Snakes
- Snails
- Vitamin K : pharmacology
- Animal
- Amphibian Venoms : chemistry
- 1-Carboxyglutamic Acid : analysis : immunology : pharmacology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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