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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03580naa a2200349 4500 | |
| 001 | oai:lup.lub.lu.se:5f9a607f-1564-40cd-ad56-156bd3ba8a77 | |
| 003 | SwePub | |
| 008 | 160401s2005 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/1515822 URI |
| 024 | 7 | a https://doi.org/10.1002/bit.205462 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Johansson, Louiseu Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)chem-ljo |
| 245 | 1 0 | a Shikimic acid production by a modified strain of E-coli (W3110.shik1) under phosphate-limited and carbon-limited conditions |
| 264 | c 2005 | |
| 264 | 1 | b Wiley,c 2005 |
| 520 | a Shikimic acid is one of several industrially interesting chiral starting materials formed in the aromatic amino acid pathway of plants and microorganisms. In this study, the physiology of a shikimic acid producing strain of Escherichia coli (derived from W31 10) deleted in aroL (shikimic acid kinase II gene), was compared to that of a corresponding control strain W31 10) under carbonand phosphate-limited conditions. For the shikimic acid producing strain (referred to as W31 10.shik1), phosphate limitation resulted in a higher yield of shikimic acid (0.059 +/- 0.012 vs. 0.024 +/- 0.005 c-mol/c-mol) and a lower yield of by-products from the shikimate pathway, when compared to carbon-limited condition. The yield of the by-product 3-clehydroshikimic acid (DHS) decreased from 0.076 +/- 0.028 to 0.022 +/- 0.001 c-mol/c-mol. Several other by-products were only detected under carbon-limited conditions. The latter group included 3-dehydroquinic acid (0.021 +/- 0.021 c-mol/c-mol), quinic acid (0.012 +/- 0.005 c-mol/c-mol), and gallic acid (0.002 +/- 0.001 c-mol/c-mol). For both strains, more acetate was produced under phosphate than the carbon-limited case. Considerable cell lysis was found for both strains but was higher for W3110.shikl ' and increased for both strains under phosphate limitation. The advantages of the latter condition in terms of an increased shikimic acid yield was thus counteracted by an increased cell lysis, which may make downstream processing more difficult. (c) 2005 Wiley Periodicals, Inc. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Kemiteknik0 (SwePub)2042 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Chemical Engineering0 (SwePub)2042 hsv//eng |
| 700 | 1 | a Lindskog, A4 aut |
| 700 | 1 | a Silfversparre, G4 aut |
| 700 | 1 | a Cimander, C4 aut |
| 700 | 1 | a Nielsen, K F4 aut |
| 700 | 1 | a Lidén, Gunnaru Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)chem-gli |
| 710 | 2 | a Avdelningen för kemiteknikb Institutionen för processteknik och tillämpad biovetenskap4 org |
| 773 | 0 | t Biotechnology and Bioengineeringd : Wileyg 92:5, s. 541-552q 92:5<541-552x 1097-0290x 0006-3592 |
| 856 | 4 | u http://dx.doi.org/10.1002/bit.20546y FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/151582 |
| 856 | 4 8 | u https://doi.org/10.1002/bit.20546 |
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