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The Lecticans of Ma...
The Lecticans of Mammalian Brain Perineural Net Are O-Mannosylated
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Pacharra, Sandra (författare)
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Hanisch, Franz-Georg (författare)
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Muehlenhoff, Martina (författare)
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Faissner, Andreas (författare)
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- Rauch, Uwe (författare)
- Lund University,Lunds universitet,Kärlväggsbiologi,Forskargrupper vid Lunds universitet,Vessel Wall Biology,Lund University Research Groups
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Breloy, Isabelle (författare)
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(creator_code:org_t)
- 2013-03-01
- 2013
- Engelska.
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 12:4, s. 1764-1771
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- O-Mannosylation is an important protein modification in brain. During the last years, a few mammalian proteins have been identified as targets of the protein-O-mannosyltransferases 1 and 2. However, these still cannot explain the high content of O-mannosyl glycans in brain and the strong brain involvement of congenital muscular dystrophies caused by POMT mutations (Walker-Warburg syndrome, dystroglycanopathies). By fractionating and analyzing the glycoproteome of mouse and calf brain lysates, we could show that proteins of the perineural net, the lecticans, are O-mannosylated, indicating that major components of neuronal extracellular matrix are O-mannosylated in mammalian brain. This finding corresponds with the high content of O-mannosyl glycans in brain as well as with the brain involvement of dystroglycanopathies. In contrast, the lectican neurocan is not O-mannosylated when recombinantly expressed in EBNA-293 cells, revealing the possibility of different control mechanisms for the initiation of O-mannosylation in different cell types.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- O-glycans
- O-mannosylation
- lecticans
- ECM
- perineural net
- O-glycosylation
- ESI-MS/MS
- MALDI-MS/MS
- glycoproteomics
- dystroglycanopathies
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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