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Sökning: onr:"swepub:oai:lup.lub.lu.se:6b8034c4-6268-447d-bd2a-f6e5e313ae29" > Orthotopic Bone For...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005000naa a2200613 4500
001oai:lup.lub.lu.se:6b8034c4-6268-447d-bd2a-f6e5e313ae29
003SwePub
008220210s2020 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/6b8034c4-6268-447d-bd2a-f6e5e313ae292 URI
0247 a https://doi.org/10.3390/ijms211972332 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Pigeot, Sébastienu University Hospital Basel4 aut
2451 0a Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage
264 c 2020-09-30
264 1b MDPI AG,c 2020
520 a Most bones of the human body form and heal through endochondral ossification, whereby hypertrophic cartilage (HyC) is formed and subsequently remodeled into bone. We previously demonstrated that HyC can be engineered from human mesenchymal stromal cells (hMSC), and subsequently devitalized by apoptosis induction. The resulting extracellular matrix (ECM) tissue retained osteoinductive properties, leading to ectopic bone formation. In this study, we aimed at engineering and devitalizing upscaled quantities of HyC ECM within a perfusion bioreactor, followed by in vivo assessment in an orthotopic bone repair model. We hypothesized that the devitalized HyC ECM would outperform a clinical product currently used for bone reconstructive surgery. Human MSC were genetically engineered with a gene cassette enabling apoptosis induction upon addition of an adjuvant. Engineered hMSC were seeded, differentiated, and devitalized within a perfusion bioreactor. The resulting HyC ECM was subsequently implanted in a 10-mm rabbit calvarial defect model, with processed human bone (Maxgraft®) as control. Human MSC cultured in the perfusion bioreactor generated a homogenous HyC ECM and were efficiently induced towards apoptosis. Following six weeks of in vivo implantation, microcomputed tomography and histological analyses of the defects revealed an increased bone formation in the defects filled with HyC ECM as compared to Maxgraft®. This work demonstrates the suitability of engineered devitalized HyC ECM as a bone substitute material, with a performance superior to a state-of-the-art commercial graft. Streamlined generation of the devitalized tissue transplant within a perfusion bioreactor is relevant towards standardized and automated manufacturing of a clinical product.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Biomaterialvetenskap0 (SwePub)304032 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Biomaterials Science0 (SwePub)304032 hsv//eng
653 a Animals
653 a Apoptosis/genetics
653 a Bone Remodeling/genetics
653 a Bone Substitutes/therapeutic use
653 a Cartilage/growth & development
653 a Cell Differentiation/genetics
653 a Extracellular Matrix/genetics
653 a Humans
653 a Mesenchymal Stem Cell Transplantation
653 a Mesenchymal Stem Cells/cytology
653 a Osteogenesis/genetics
653 a Rabbits
653 a Skull/growth & development
653 a Tissue Engineering/methods
653 a Tissue Scaffolds
653 a Wound Healing/genetics
7001 a Bourgine, Paul Emileu Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Molecular Skeletal Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine,University Hospital Basel4 aut0 (Swepub:lu)pa2431bo
7001 a Claude, Jaquieryu University Hospital Basel4 aut
7001 a Scotti, Celesteu Novartis Institutes for BioMedical Research, Inc.,Istituto Ortopedico Galeazzi4 aut
7001 a Papadimitropoulos, Adamu University Hospital Basel4 aut
7001 a Todorov, Atanasu University Hospital Basel4 aut
7001 a Epple, Christianu University Hospital Basel4 aut
7001 a Peretti, Giuseppe Mu Istituto Ortopedico Galeazzi,University of Milan4 aut
7001 a Martin, Ivanu University Hospital Basel4 aut
7102 a University Hospital Baselb Molekylär skelettbiologi4 org
7730 t International Journal of Molecular Sciencesd : MDPI AGg 21:19q 21:19x 1422-0067
8564 u http://dx.doi.org/10.3390/ijms21197233x freey FULLTEXT
8564 u https://www.mdpi.com/1422-0067/21/19/7233/pdf
8564 8u https://lup.lub.lu.se/record/6b8034c4-6268-447d-bd2a-f6e5e313ae29
8564 8u https://doi.org/10.3390/ijms21197233

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