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Immobilization of C...
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Aragao, RosaLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
(författare)
Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production
- Artikel/kapitelEngelska2014
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LIBRIS-ID:oai:lup.lub.lu.se:6fa224fa-11f5-4381-adcd-1d161123b896
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https://lup.lub.lu.se/record/4376574URI
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https://doi.org/10.1016/j.procbio.2013.09.027DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3-5 times in partial or completely fresh medium, reaching a maximum butanol concentration in the broth of 18.2 g/l and yield of 0.41 (g/g) in one of the cycles. The use of cells based cryogels can be a good alternative for improvement of acetone-butanol-ethanol (ABE) process as cells are immobilized in a macroporous structure with low limitations for mass transfer with potential for high yield production. (C) 2013 Elsevier Ltd. All rights reserved.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Zaushitsyna, OksanaLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)medk-ob0
(författare)
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Berillo, DmitryLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-dbo
(författare)
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Scaccia, NazarenoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-nos
(författare)
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Mattiasson, BoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-bma
(författare)
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Kirsebom, HaraldLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)med-hko
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BioteknikCentrum för tillämpade biovetenskaper
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Sammanhörande titlar
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Ingår i:Process Biochemistry: Elsevier BV49:1, s. 10-181873-32981359-5113
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