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Chemical genetic sc...
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Ducommun, SergeSwiss Federal Institute of Technology
(författare)
Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:7338f3d3-1b26-4e49-b7c4-2a6f1953b560
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https://lup.lub.lu.se/record/7338f3d3-1b26-4e49-b7c4-2a6f1953b560URI
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https://doi.org/10.1016/j.cellsig.2019.02.001DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:140567797URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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AMP-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis, acting as a sensor of energy and nutrient status. As such, AMPK is considered a promising drug target for treatment of medical conditions particularly associated with metabolic dysfunctions. To better understand the downstream effectors and physiological consequences of AMPK activation, we have employed a chemical genetic screen in mouse primary hepatocytes in an attempt to identify novel AMPK targets. Treatment of hepatocytes with a potent and specific AMPK activator 991 resulted in identification of 65 proteins phosphorylated upon AMPK activation, which are involved in a variety of cellular processes such as lipid/glycogen metabolism, vesicle trafficking, and cytoskeleton organisation. Further characterisation and validation using mass spectrometry followed by immunoblotting analysis with phosphorylation site-specific antibodies identified AMPK-dependent phosphorylation of Gapex-5 (also known as GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1)) on Ser902 in hepatocytes and starch-binding domain 1 (STBD1) on Ser175 in multiple cells/tissues. As new promising roles of AMPK as a key metabolic regulator continue to emerge, the substrates we identified could provide new mechanistic and therapeutic insights into AMPK-activating drugs in the liver.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Deak, MariaSwiss Federal Institute of Technology
(författare)
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Zeigerer, AnjaHelmholtz Zentrum München,University Hospital Heidelberg,German Center for Diabetes Research
(författare)
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Göransson, OlgaLund University,Lunds universitet,Proteinfosforylering,Forskargrupper vid Lunds universitet,Protein Phosphorylation,Lund University Research Groups(Swepub:lu)medk-ogo
(författare)
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Seitz, SusanneGerman Center for Diabetes Research,University Hospital Heidelberg,Helmholtz Zentrum München
(författare)
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Collodet, CaterinaSwiss Federal Institute of Technology
(författare)
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Madsen, Agnete B.University of Copenhagen
(författare)
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Jensen, Thomas E.University of Copenhagen
(författare)
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Viollet, BenoitParis Descartes University,Institut Cochin
(författare)
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Foretz, MarcInstitut Cochin,Paris Descartes University
(författare)
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Gut, PhilippSwiss Federal Institute of Technology
(författare)
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Sumpton, DavidBeatson Institute for Cancer Research
(författare)
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Sakamoto, KeiSwiss Federal Institute of Technology
(författare)
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Swiss Federal Institute of TechnologyHelmholtz Zentrum München
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Cellular Signalling: Elsevier BV57, s. 45-570898-65681873-3913
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Till lärosätets databas
- Av författaren/redakt...
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Ducommun, Serge
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Deak, Maria
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Zeigerer, Anja
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Göransson, Olga
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Seitz, Susanne
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Collodet, Cateri ...
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visa fler...
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Madsen, Agnete B ...
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Jensen, Thomas E ...
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Viollet, Benoit
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Foretz, Marc
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Gut, Philipp
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Sumpton, David
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Sakamoto, Kei
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Cellular Signall ...
- Av lärosätet
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Lunds universitet
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Karolinska Institutet